超支化聚缩水甘油-羟喜树碱药物递送系统对人腺样囊性癌的体外抑制作用

doi:10.3969/j.issn.1005-4979.2015.03.006

《口腔颌面外科杂志》 ›› 2015, Vol. 25 ›› Issue (3): 190-. doi: 10.3969/j.issn.1005-4979.2015.03.006

超支化聚缩水甘油-羟喜树碱药物递送系统对人腺样囊性癌的体外抑制作用

马嘉磊1，吕明明2，孙坚2，周小康1，廖建兴1

1. 同济大学口腔医学院口腔生物医学及转化医学实验室，上海 200072；2. 上海第九人民医院口腔颌面外科，上海 200011

出版日期:2015-06-28 发布日期:2015-12-01
通讯作者: 廖建兴，主任医师 E-mail:ljx@tongji.edu.cn
作者简介:马嘉磊（1989—）,男，上海人,硕士研究生. E-mail: shmily182182@163.com
基金资助:
上海市科学技术委员会科研计划项目（12nm0503002）

Inhibition Effects of Hyperbranched Polyglycidol-HA Drug Delivery System on Human Adenoid Cystic Carcinoma in Vitro

MA Jia-lei1, LU Ming-ming2, SUN Jian2, ZHOU Xiao-kang1, LIAO Jian-xing1

1. Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai 200072; 2. Department of Oral and Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai 200011, China

Online:2015-06-28 Published:2015-12-01

摘要/Abstract

摘要： 目的：研究超支化聚缩水甘油-羟喜树碱药物递送系统（biodegradable hyperbranched polyglycerol-hydroxy camptothecin, dHPG-HA）对体外培养的人腺样囊性癌（高肺转移）细胞（salivary adenoid cystic carcinoma-lung metastases, SACC-LM）的抑制作用。方法：细胞计数试剂盒（cell counting kit, CCK8）检测药物浓度为0.001、0.01、0.1、1、10、100 μg/mL的羟喜树碱（hydroxy camptothecin, HA）和含等量HA的dHPG-HA，在24、48 h对肿瘤细胞的抑制作用；流式细胞仪检测浓度为0.001、0.01、0.1、1、10 μg/mL的dHPG-HA是否能诱导SACC-LM细胞凋亡。结果：HA和dHPG-HA对SACC-LM细胞增殖均有明显抑制作用，且作用呈剂量依赖性和时间依赖性；dHPG-HA对SACC-LM细胞的细胞毒性明显低于HA；dHPG-HA能诱导SACC-LM细胞凋亡。结论：dHPG的构建能达到使药物HA缓释的作用并降低其细胞毒性。

关键词: 腺样囊性癌（高肺转移）细胞, 超支化聚缩水甘油?鄄羟喜树碱药物递送系统, 抑制作用, 细胞凋亡

Abstract: Objective: To investigate the inhibition effects of hyperbranched polyglycidol-hydroxy camptothecin (dHPG-HA) drug delivery system on cultured human adenoid cystic carcinoma (high lung metastasis) cells (SACC-LM). Methods: SACC-LM cells cultured in vitro were treated with different concentrations of hydroxy camptothecin (HA) and (dHPG-HA) which contained equal amounts of HA, and the proliferation inhibition of SACC-LM cells was detected with cell counting kit assay (CCK8). Apoptosis of SACC-LM cells treated by various concentrations of HA and dHPG-HA was ascertained by flow cytometry. Results: The proliferation of SACC-LM cells was significantly reduced. And the effect was dose-dependent and time-dependent. In addition, the cytotoxicity of dHPG-HA on SACC-LM cells was obviously lower than free HA. Furthermore, dHPG-HA could induce apoptosis of SACC-LM cells effectively. In a word, the construction of drug carrier (dHPG) on HA provided sustained release of drugs and reduced its cytotoxicity.

Key words: salivary adenoid cystic carcinoma-lung metastases cell, biodegradable hyperbranched polyglycerol-hydroxy camptothecin, inhibition, apoptosis

中图分类号:

R782
R739.8

马嘉磊1，吕明明2，孙坚2，周小康1，廖建兴1. 超支化聚缩水甘油-羟喜树碱药物递送系统对人腺样囊性癌的体外抑制作用[J]. 《口腔颌面外科杂志》, 2015, 25(3): 190-.

MA Jia-lei1, LU Ming-ming2, SUN Jian2, ZHOU Xiao-kang1, LIAO Jian-xing1. Inhibition Effects of Hyperbranched Polyglycidol-HA Drug Delivery System on Human Adenoid Cystic Carcinoma in Vitro[J]. 《Journal of Oral and Maxillofacial Surgery》, 2015, 25(3): 190-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2015/V25/I3/190

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超支化聚缩水甘油-羟喜树碱药物递送系统对人腺样囊性癌的体外抑制作用

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