摘要：

目的：探讨沉默信息调节因子3（sirtuin3，SIRT3）对舌癌细胞凋亡的影响。方法：采用小干扰RNA（small interfere RNA，siRNA）沉默舌癌细胞中的SIRT3。MTT法检测SIRT3沉默对舌癌细胞增殖活力的影响；TUNEL染色检测SIRT3沉默对舌癌细胞凋亡的影响；酶联免疫吸附实验（enzyme linked immunosorbent assay, ELISA）检测SIRT3沉默对舌癌细胞半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）活性的影响；蛋白质免疫印迹法（Western blot）检测SIRT3沉默对舌癌细胞Caspase-3和多聚二磷酸腺苷核糖聚合酶（poly ADP-ribose polymerase, PARP）表达的影响。结果：SIRT3沉默有效，在SIRT3沉默后观察到舌癌细胞的增殖活力降低，细胞凋亡增加，Caspase-3活性增加，Caspase-3和PARP表达增加。沉默SIRT3前后，舌癌细胞的增殖活力、TUNEL阳性细胞比例、Caspase-3的活性值以及Caspase-3和PARP表达水平差异有统计学意义（P<0.05）。结论：SIRT3沉默可促进舌癌细胞凋亡。

关键词: 沉默信息调节因子3, 舌鳞状细胞癌, 细胞凋亡

Abstract:

Objective: To explore the role of sirtuin3(SIRT3) in regulating the apoptosis of tongue squamous cell carcinoma (TSCC) cells in vitro. Methods: The knockdown of SIRT3 of TSCC cell lines were accomplished through small interfering RNA (siRNA). The proliferation activity of the TSCC cells was examined by MTT assay; the apoptosis of the TSCC cell lines was tested using TUNEL staining; the Caspase-3 activity of the cells was analyzed by enzyme-linked immunosorbent assay (ELISA); and the expression of Caspase-3 and PARP of 2 squamous cell carcinoma (SCC) cells was determined with Western blotting. Results: SIRT3 knockdown in TSCC cells significantly lowered the proliferation activity, increased the number of TUNEL-positive cells, enhanced the Caspase-3 activity, and the expression of Caspase-3 and PARP were significantly high in the two SCC cell lines than the control group. The proliferation activity, the number of TUNEL-positive cell, the activity of Caspase-3, and the expression of Caspase-3 and PARP in TSCC cells were significantly different before and after modulation of SIRT3 expression (P<0.05). Conclusion: Knockdown of SIRT3 plays a positive role for apoptosis of SCC cells in vitro.

Key words: sirtuin3（SIRT3）, tongue squamous cell carcinoma, apoptosis

中图分类号: 

• R782

• R739.8