摘要： 目的：探究过表达Tafazzin（TAZ）对口腔舌鳞癌CAL-27、SCC-15细胞增殖迁移侵袭的影响及其作用机制。方法：利用成功过表达TAZ的慢病毒载体转染CAL-27、SCC-15细胞，分为过表达TAZ组（overexpression TAZ, OE TAZ）和对照组(negative control, NC)。用CCK-8实验检测细胞增殖；用划痕实验检测细胞的迁移；用Transwell实验检测细胞侵袭。相关基因的mRNA水平和蛋白水平分别利用实时荧光定量PCR（qRT-PCR）和Western blot检测。结果：qRT-PCR和Western blot结果显示，过表达TAZ病毒转染成功；过表达组较对照组增殖升高，迁移侵袭明显增强；增殖相关蛋白p-Erk，p-Akt表达量升高，上皮间充质转化（epithelial-mesenchymal transition，EMT）相关蛋白E-钙黏连蛋白（E-Cadherin）降低，波形蛋白（Vimentin）升高。结论：过表达TAZ可能通过影响p-Erk、p-Akt、E-Cadherin、Vimentin的表达促进口腔舌鳞癌CAL-27、SCC-15细胞的增殖迁移和侵袭。

关键词: Tafazzin（TAZ）, 口腔鳞癌, 细胞增殖, 细胞迁移, 细胞侵袭

Abstract: Objective: To investigate the effects of overexpressing TAZ on proliferation, migration and invasion of CAL-27、SCC-15 cells and the underlying molecular mechanism. Methods: Tongue squamous carcinoma cells(CAL-27、SCC-15) were used in this study. Lentivirus packaging cells were transfected with LV5-homo-TAZ and LV5-NC, and divided cells into overexpression TAZ (OE TAZ) group and negative control (NC) group. Cell proliferation was determined using CCK-8 assays. Cell migration was measured by scracth assay and cell invasion was measured by transwell assay. The mRNA and related protein levels were detected by qRT-PCR and western blot assays. Results: Western Blot and qRT-PCR analysis revealed successful TAZ overexpression. In overexpression TAZ group, the proliferation rate increased, and cell migration and invasion increased significantly compared to NC group. Proliferation-related proteins increased, such as p-Erk and p-Akt. Epithelial-mesenchymal transition(EMT)-related protein as E-Cadherin decreased while Vimentin was increased. Conclusion: Overexpression TAZ might promote proliferation, migration and invasion by affecting the protein expressions of p-Erk、p-Akt、E-Cadherin、Vimentin in tongue squamous carcinoma CAL-27、SCC-15 cells.

Key words: TAZ, cell proliferation, cell migration, cell invasion

中图分类号: 

• R856.78