摘要： 目的：探讨双膦酸盐相关性颌骨坏死病损组织中血管内皮生长因子（VEGF）的表达，微血管生成及其与临床分期的关系。方法：采用S-P免疫组织化学染色法检测24例双膦酸盐相关性颌骨坏死（观察组），17例颌骨隆突（对照组）组织中VEGF的表达及微血管密度（MVD）。结果：观察组的VEGF表达阳性率为25%（6/24），MVD为（2.04±0.81） mm2，显著低于对照组的VEGF阳性表达率（64.7%）和MVD值（7.06±1.71） mm2（P＜0.05），且VEGF与MVD值呈正相关（P＜0.05）。观察组按美国口腔颌面外科医师协会确立的标准进行分期，0~Ⅰ期14例，Ⅱ~Ⅲ期10例，二组在VEGF的阳性表达率、MVD无显著性差异（P＞0.05），与临床分期不相关。结论：双膦酸盐相关性颌骨坏死病损区微血管生成受到抑制，且与血管内皮生长因子低度表达相关。

关键词: 双膦酸盐相关性颌骨坏死, 血管内皮生长因子, 微血管密度, 免疫组织化学染色

Abstract: Objective: To investigate the expression of vascular endothelial growth factor (VEGF) and micro-vascular angiogenesis in bisphosphonate-associated osteonecrosis of the jaws(BRONJ), and their relationships with clinical stages of BRONJ. Methods: S-P immunohistochemical staining was used to detect the expression of VEGF and microvascular density (MVD) in the lesions of 24 patients with bisphosphonate-associated jaw necrosis (observation group) and 17 patients with jaw juga (control group). Results: The positive rate of VEGF expression in observation group (25%) was significantly lower than that in control group (64.7%), as well as the MVD (2.04±0.81/mm2 vs 7.06±1.71/mm2) (P<0.05), and the level of VEGF was positively correlated with the MVD(P<0.05). Observation group was divided into two groups according to the staging standard of American association of oral and maxillofacial surgeons, one group included 14 cases of stage 0~Ⅰand another group included 10 cases of stage Ⅱ~Ⅲ. There was no significant difference between these two groups in the positive expression rate of VEGF and MVD(P＞0.05). Conclusion: Micro-vascular angiogenesis in the lesions of bisphosphonate-associated jaw bone necrosis is inhibited, which may be correlated with low expression of vascular endothelial growth factor.

Key words: bisphosphonate related osteonecrosis of the jaw, vascular endothelial growth factor, microvascular density, immunohistochemical staining

中图分类号: 

• R782