LincRNA-EPS对牙周炎小鼠肠道菌群影响的实验研究

doi:10.3969/j.issn.1005-4979.2023.03.002

摘要/Abstract

摘要：

目的：探究基因间长链非编码RNA-EPS（long intergenic noncoding RNA-EPS, lincRNA-EPS）对牙周炎小鼠肠道菌群的影响。方法：选用8周龄雄性C57BL/6野生型小鼠和lincRNA-EPS基因敲除小鼠，分为野生型对照组（N组即group 1, n=6）、敲除型对照组（Nplus组即group 2, n=6）、野生型牙周炎组（P组即group 3, n=6）和敲除型牙周炎组（Pplus组即group 4, n=5）。对group 3及group 4小鼠采用丝线结扎法构建牙周炎模型，结扎10 d后收集各组小鼠粪便样品，采用16S rDNA测序技术分析各组小鼠肠道菌群的组成和结构。使用微型CT（micro-CT）检测野生型小鼠和lincRNA-EPS基因敲除小鼠上颌磨牙区的形态。结果：16S rDNA测序结果显示，lincRNA-EPS基因敲除牙周炎小鼠与野生型牙周炎小鼠的肠道菌群α多样性差异不明显（P>0.05），2组β多样性差异具有统计学意义（P<0.05）。在门水平及属水平上，两者物种组成及丰度差异具有统计学意义（P<0.05）。结论：lincRNA-EPS在一定程度上能够改变牙周炎小鼠肠道菌群的构成，其具体机制有待进一步研究。

关键词: 长链非编码RNA-EPS, 牙周炎, 肠道菌群, 高通量测序, 16S rDNA

Abstract:

Objective: To investigate the effect of long intergenic noncoding RNA-EPS (lincRNA-EPS) on intestinal flora in mice with periodontitis. Methods: 8-week-old male C57BL/6 wild-type mice and lincRNA-EPS knockout mice were selected and divided into wild-type control group (group N, i.e. group 1, n=6), knockout control group (group Nplus, i.e. group 2, n=6), wild-type periodontitis group (group P, i.e. group 3, n=6), and knockout periodontitis group (group Pplus, i.e. group 4, n=5). The periodontitis model was established in mice group 3 and group 4 by silk ligation, and fecal samples were collected from each group after 10 d of ligation, then 16S rDNA sequencing was applied to analyze the composition and structure of the intestinal flora of each group. Micro-CT was used to detect the morphology of the maxillary molar region in each group. Results: 16S rDNA sequencing showed that the lincRNA-EPS knockout mice had no significant difference in the α diversity of intestinal flora compared with wild-type periodontitis mice (P>0.05), and the β diversity was significantly different (P<0.05). The species composition and their abundance were significantly different at the phylum and genus levels (P<0.05). Conclusion: lincRNA-EPS can change the composition of intestinal flora in mice with periodontitis to some extent. Further studies are required to develop conclusions regarding the performance.

Key words: long intergenic noncoding RNA-EPS, periodontitis, intestinal flora, high-throughput sequencing, 16S rDNA

中图分类号:

R782

陈栋怡, 苏俭生. LincRNA-EPS对牙周炎小鼠肠道菌群影响的实验研究[J]. 《口腔颌面外科杂志》, 2023, 33(3): 138-147.

CHEN Dongyi, SU Jiansheng. LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study[J]. 《Journal of Oral and Maxillofacial Surgery》, 2023, 33(3): 138-147.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2023/V33/I3/138

图/表 11

参考文献 18

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菌种	group 2	group 1	P值
Bacteroidetes	0.494±0.056	0.558±0.073	0.507
Firmicutes	0.351±0.055	0.402±0.077	0.597
Proteobacteria	0.097±0.006	0.015±0.012^①	<0.001
Verrucomicrobia	0.033±0.006	0.002±0.001^①	0.001
Tenericutes	0.007±0.001	0.011±0.005	0.384
Cyanobacteria	0.007±0.001	0.004±0.003	0.401
Actinobacteria	0.005±0.001	0.003±0.000^①	0.030
Saccharibacteria	0.003±0.001	0.001±0.000^①	0.043
Fusobacteria	0.000±0.000	0.003±0.003	0.272
Deferribacteres	0.002±0.001	0.000±0.000^①	0.011

菌种	group 2	group 1	P值
Bacteroidetes	0.494±0.056	0.558±0.073	0.507
Firmicutes	0.351±0.055	0.402±0.077	0.597
Proteobacteria	0.097±0.006	0.015±0.012^①	<0.001
Verrucomicrobia	0.033±0.006	0.002±0.001^①	0.001
Tenericutes	0.007±0.001	0.011±0.005	0.384
Cyanobacteria	0.007±0.001	0.004±0.003	0.401
Actinobacteria	0.005±0.001	0.003±0.000^①	0.030
Saccharibacteria	0.003±0.001	0.001±0.000^①	0.043
Fusobacteria	0.000±0.000	0.003±0.003	0.272
Deferribacteres	0.002±0.001	0.000±0.000^①	0.011

菌种	group 4	group 3	P值
Bacteroidetes	0.650±0.056	0.620±0.044	0.726
Firmicutes	0.278±0.044	0.293±0.046	0.816
Proteobacteria	0.020±0.005	0.054±0.050	0.604
Verrucomicrobia	0.041±0.009	0.008±0.003^①	0.005
Fusobacteria	0.000±0.000	0.019±0.019	0.331
Actinobacteria	0.003±0.001	0.003±0.000	0.824
Tenericutes	0.002±0.000	0.001±0.001	0.716
Cyanobacteria	0.002±0.001	0.000±0.000^①	0.014
Deferribacteres	0.002±0.001	0.000±0.000	0.088
Saccharibacteria	0.000±0.000	0.000±0.000	0.234

菌种	group 4	group 3	P值
Bacteroidetes	0.650±0.056	0.620±0.044	0.726
Firmicutes	0.278±0.044	0.293±0.046	0.816
Proteobacteria	0.020±0.005	0.054±0.050	0.604
Verrucomicrobia	0.041±0.009	0.008±0.003^①	0.005
Fusobacteria	0.000±0.000	0.019±0.019	0.331
Actinobacteria	0.003±0.001	0.003±0.000	0.824
Tenericutes	0.002±0.000	0.001±0.001	0.716
Cyanobacteria	0.002±0.001	0.000±0.000^①	0.014
Deferribacteres	0.002±0.001	0.000±0.000	0.088
Saccharibacteria	0.000±0.000	0.000±0.000	0.234

菌种	group 2	group 1	P值
other	0.419±0.023	0.396±0.030	0.654
uncultured_Bacteroidales_bacterium	0.190±0.020	0.061±0.016^①	<0.001
Bacteroides	0.009±0.002	0.134±0.020^①	<0.001
Ruminococcaceae_UCG-014	0.044±0.011	0.068±0.020	0.300
Lachnospiraceae_NK4A136_group	0.058±0.021	0.036±0.012	0.451
Alloprevotella	0.017±0.002	0.060±0.014^①	0.003
Allobaculum	0.028±0.005	0.030±0.009	0.865
mouse_gut_metagenome	0.008±0.001	0.030±0.009^①	0.016
Parabacteroides	0.002±0.001	0.034±0.007^①	<0.001
Akkermansia	0.033±0.006	0.002±0.001^①	<0.001

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