摘要： 目的： 通过FX-4000柔性基底膜加载系统实现舌鳞癌Tca8113细胞的体外拉伸加载，观察拉伸刺激对Tca8113细胞侵袭力变化及基质金属蛋白酶-2（MMP-2）表达的影响,以探讨拉伸刺激在舌鳞癌侵袭转移中的作用。方法： 选取2%拉伸幅度、频率2 Hz拉伸加载Tca8113细胞，以在相同培养小室中静态培养细胞作为对照。细胞在拉伸加载后行MMP-2免疫组织化学SP染色，用图像分析软件进行定量分析；Transwell小室肿瘤侵袭力实验测定Tca8113细胞侵袭力。结果： 拉伸组细胞的侵袭力和MMP?鄄2表达均高于对照组细胞，差异有统计学意义（P<0.05）；不同拉伸时间下各拉伸组间MMP-2表达无统计学意义(P>0.05)，侵袭力变化有统计学意义（P<0.05）。结论： 拉伸刺激可以促进舌鳞癌细胞MMP-2的表达和侵袭力变化，在舌鳞癌侵袭转移中起着一定的作用。
关键词:
拉伸,
舌鳞癌,
侵袭力,
基质金属蛋白酶-2
Abstract: Objective: To observe tensile stimulate expressing matrix metalloproteinases-2 (MMP-2) and to investigate the effort of tensile stimulation in the invasion capacity and metastasis of Tca8113 cells in vitro. Methods: 2% 2 Hz strain tensile were loaded on the Tca8113 cells while unstrained cells were used as a control. The expression of MMP-2 was studied with immunohistochemical techniques and image software for quantitative analysis, the invasion capacity of Tca8113 cells was determined by Transwell in vitro. Results: The expression of MMP-2 and the invasion capacity of the strained group was obviously higher than the unstrained group (P<0.05). The expression of MMP-2 didn’t change as the straining time changed (P>0.05), but the invasion capacity did (P<0.05). Conclusion: Our results suggest that tensile stimulation enhanced the expression of MMP-2 in Tca8113 cell line and may be involved in the potential of tumor invasion and metastasis.
Key words:
stretch,
tongue squamous cell carcinoma,
invasion,
MMP-2
中图分类号:
孙明星， 薛晓凤， 任富凯， 范亚伟. 拉伸刺激对舌鳞癌细胞侵袭力及MMP-2表达的影响[J]. 《口腔颌面外科杂志》, 2012, 22(1): 27-29.
SUN Ming-xing, XUE Xiao-feng, REN Fu-kai, FAN Ya-wei. Effort of Tensile Stimulation on Expression of MMP-2 and the Invasion Capacity in Human Tongue Squamous Cell Carcinoma[J]. 《Journal of Oral and Maxillofacial Surgery》, 2012, 22(1): 27-29.