Nestin阳性细胞中DMP1过表达对小鼠软骨细胞分化的影响

doi:10.3969/j.issn.1005-4979.2018.03.004

《口腔颌面外科杂志》

Nestin阳性细胞中DMP1过表达对小鼠软骨细胞分化的影响

张书凡，潘敏，万会璇，孙瑶

上海牙组织修复与再生工程技术中心，同济大学口腔医学院，上海 200072

出版日期:2018-06-28 发布日期:2019-11-28
通讯作者: 孙瑶，教授. E-mail: sunyao919@126.com
作者简介:张书凡（1991—），男，广东人，硕士研究生. E-mail: zhangshufans@126.com
基金资助:
国家自然基金（81470715、81771043）；国家重点研发计划（2016YFC1102705）；上海市卫生系统优秀学科带头人计划课题（2017BR009）

Overexpression of DMP1 in Nestin Positive Cells Promotes Chondrocyte Differentiation in Mice

ZHANG Shu-fan, PAN Min, WAN Hui-xuan, SUN Yao

School of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China

Online:2018-06-28 Published:2019-11-28

摘要/Abstract

摘要： 目的：细胞外基质蛋白对软骨发育有重要调控作用。近期研究发现DMP1是软骨发育过程中特异性表达的基质蛋白，但其对软骨细胞分化的作用尚未清楚。Nestin阳性细胞是间充质干细胞中重要的一个亚群，我们通过在Nestin阳性细胞中过表达DMP1，研究DMP1对软骨细胞分化的影响。方法：以Nestin为启动子构建DMP1转基因小鼠模型，通过组织学切片染色观察小鼠关节软骨结构和细胞形态的变化，实时荧光定量PCR分析相关基因的表达差异。结果：DMP1转基因小鼠关节软骨层增厚，软骨细胞数量增加，Sox9、Col Ⅱ、Col X、Acan等软骨细胞分化相关基因表达上调。结论：DMP1能够促进小鼠Nestin阳性的软骨细胞分化，提示DMP1对软骨发育可能具有调控作用。

关键词: DMP1, Nestin, 软骨细胞, 关节软骨, 软骨发育

Abstract: Objective: To study whether the overexpressed Dentin Matrix Protein (dmp1 )in nestin positive cells was related to the promotion of chondrocyte differentiation. Methods: DMP1 transgene mice model was constructed by nestin promoter. Histological section and staining were used to observe the change of mice articular cartilage structure and cell morphology. Real-time quantitative PCR were performed to analyze the differences of related gene expression. Results: The thickness of DMP1 transgene mice articular cartilage increased, hypertrophic chondrocyte number increased and gene expression of Sox 9, Col Ⅱ, Col X, and Acan was up-regulated. Conclusion: DMP1 promotes nestin positive chondrocyte differentiation in mice, suggests DMP1 may regulate cartilage development.

Key words: DMP1, Nestin, chondrocyte, articular cartilage, cartilage development

中图分类号:

R856.78

张书凡，潘敏，万会璇，孙瑶. Nestin阳性细胞中DMP1过表达对小鼠软骨细胞分化的影响[J]. 《口腔颌面外科杂志》, doi: 10.3969/j.issn.1005-4979.2018.03.004.

ZHANG Shu-fan, PAN Min, WAN Hui-xuan, SUN Yao. Overexpression of DMP1 in Nestin Positive Cells Promotes Chondrocyte Differentiation in Mice[J]. 《Journal of Oral and Maxillofacial Surgery》, doi: 10.3969/j.issn.1005-4979.2018.03.004.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2018/V28/I3/142

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Nestin阳性细胞中DMP1过表达对小鼠软骨细胞分化的影响

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