骨保护素基因敲除对老年小鼠关节软骨的影响

doi:10.3969/j.issn.1005-4979.2016.04.004

《口腔颌面外科杂志》 ›› 2016, Vol. 26 ›› Issue (4): 251-255. doi: 10.3969/j.issn.1005-4979.2016.04.004

骨保护素基因敲除对老年小鼠关节软骨的影响

张立立，孙瑶

1. 同济大学口腔医学院基础教研室，上海 200072；
2. 同济大学附属口腔医院种植科，上海牙组织修复与再生工程技术研究中心，上海 200072

收稿日期:2016-02-22 修回日期:2016-04-06 出版日期:2016-08-28 发布日期:2016-09-24
通讯作者: 孙瑶，教授. E-mail: yaosun@tongji.edu.cn;sunyao@126.com
作者简介:张立立（1989—），女，河北人，硕士研究生. E-mail: 952689320@qq.com
基金资助:
国家自然科学基金（NSFC81300840）；国家自然科学基金（NSFC81470715）；上海市卫生系统新优青计划（XYQ2013080）；国家重点研发计划课题（2016YFC1102705）；高等学校博士学科专项科研基金（20130072120024）；上海市卫生和计划生育委员会科研课题（XYQ2013080）

Effect of Osteoprotegerin Knockout in Aged Mice on the Articular Cartilage

ZHNAG Li-li, SUN Yao

1. Department of Basic Oral Science, School of Stomatology, Tongji University, Shanghai 200072;
2. Department of Dental Implantology, Hospital of Stomatology, Tongji University, Shanghai
Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China

Received:2016-02-22 Revised:2016-04-06 Online:2016-08-28 Published:2016-09-24

摘要/Abstract

摘要： 目的：研究骨保护素（osteoprotegerin, OPG）在老年小鼠关节软骨增龄性变化中的作用及意义。方法：取鼠龄为1年的骨保护素基因敲除（opg-knockout, Opg-KO）型小鼠和野生型（wildtype，WT）小鼠的长骨组织，分别进行X-ray、micro-CT检查，并行组织学染色观察关节软骨组织形态学的改变；行甲苯胺蓝染色，观察关节软骨蛋白聚糖的改变；行免疫组化染色，观察关节软骨Ⅱ型胶原表达情况。结果：与WT小鼠相比，Opg-KO 小鼠关节表面粗糙，边缘性骨赘增多，软骨下骨的骨密度增高，关节软骨表面扁平塌陷，软骨细胞明显减少，排列紊乱，蛋白聚糖显著丢失，Ⅱ型胶原表达明显减少。结论：Opg-KO 小鼠发生了严重骨关节炎，骨保护素对于维持老年小鼠关节软骨结构的完整有重要作用。

关键词: , 骨保护素, , , 关节软骨, , , Ⅱ型胶原

Abstract: Objective: To investigate the roles of osteoprotegerin (OPG) in the age related changes of articular cartilage in old mice. Methods: The long bone tissues of one-year-old Opg-Knockout(Opg-KO) mice and wildtype mice (WT mice) were examed by X-ray, micro-CT, HE staining to observe the histological changes of articular cartilage; toluidine blue staining was used to exam the changes of proteoglycan in articular cartilage; immunohistochemistry was used to observe the expression level of collagen Ⅱ in articular cartilage. Results: Compared with WT mice, specific changes in Opg-KO mice articular cartilage included rough joint surface, increased marginal osteophytes, increased bone density of the subchondral bone, flattened and collapsed articular cartilage surface, decreased number of chondrocytes, disorganized chondrocytes, loss of proteoglycan, decreased expression of collagen Ⅱ. Conclusion: Opg-KO mice develope severe osteoarthritis, and osteoprotegerin is important for maintaining the structural integrity of articular cartilage in aged mice.

Key words: osteoprotegerin, articular cartilage, collagen Ⅱ

中图分类号:

R782.6

张立立，孙瑶. 骨保护素基因敲除对老年小鼠关节软骨的影响[J]. 《口腔颌面外科杂志》, 2016, 26(4): 251-255.

ZHNAG Li-li, SUN Yao. Effect of Osteoprotegerin Knockout in Aged Mice on the Articular Cartilage[J]. 《Journal of Oral and Maxillofacial Surgery》, 2016, 26(4): 251-255.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2016/V26/I4/251

参考文献

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[8] Wang B, Jin H, Shu B, et al. Chondrocytes-specific expression of osteoprotegerin modulates osteoclast formation in metaphyseal bone[J]. Sci Rep, 2015, 5:13667.

[9] Shimizu S, Asou Y, Itoh S, et al. Prevention of cartilage destruction with intraarticular osteoclastogenesis inhibitory factor/osteoprotegerin in a murine model of osteoarthritis[J]. Arthritis Rheum, 2007, 56(10):3358-3365.

[10] Sagar DR, Ashraf S, Xu L, et al. Osteoprotegerin reduces the development of pain behaviour and joint pathology in a model of osteoarthritis[J]. Ann Rheum Dis, 2014, 73(8):1558-1565.

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[15] Liu YD, Liao LF, Zhang HY, et al. Reducing dietary loading decreases mouse temporomandibular joint degradation induced by anterior crossbite prosthesis[J]. Osteoarthritis Cartilage, 2014, 22(2):302-312.

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