摘要： 目的：探讨在不同强度及不同时间的静压力作用下，髁突软骨细胞中MMP-13表达的变化及意义。方法：体外分离培养SD大鼠髁突软骨细胞，用甲苯胺蓝及Ⅱ型胶原免疫细胞化学染色进行鉴定；分别用0、50、100、150、200 kPa静压力进行2 h的加压处理。另外在200 kPa静压力下，分别对髁突软骨细胞进行0、1、2、4、8和12 h的加压，采用Western blot免疫印迹及实时荧光定量PCR，分析髁突软骨细胞在不同强度及不同时间的静压力加载后，MMP-13的mRNA和蛋白表达变化。结果：在50~200 kPa的静压力范围内，随着压力的增大，MMP-13蛋白及mRNA表达水平均呈下降趋势,实验组表达水平均降至对照组（0 kPa组）以下，且差异有统计学意义（P<0.05)。在200 kPa的静压力下，随着加压时间的延长，MMP-13表达呈先上升后下降的趋势，且4 h实验组达最高水平（P<0.05)。结论：髁突软骨细胞对压力微环境的改变具有较好的适应能力，压力的改变可能影响颞下颌关节的适应性改建。
关键词:
髁突软骨细胞,
,
,
静压力,
,
,
MMP-13
Abstract: Objective: To investigate the effect of the static pressure on the mRNA and protein expression of MMP-13 in condylar chondrocytes. Methods: Condylar chondrocytes were dissected and cultured from the TMJ of 3-week-old SD rat in vitro. The chondrocytes of P3 were identified and were subjected to different static pressure of 0, 50, 100, 150 and 200 kPa respectively for 2 h, while the monolayer of condylar chondrocytes were subjected to static pressure of 200 kPa for different time (0, 1, 2, 4, 8 and 12 h). Expression of MMP-13 was examined by Western blot and quantitative real-time PCR. Results: The static pressure with a magnitude from 50 to 200 kPa can reduce the expression of MMP-13 of condylar chondrocytes, and has a significant difference (P<0.05). Under static pressure of 200 kPa, with the compression time extended, the expression of MMP-13 first increased and then decreased, and up to the highest level at 4 h(P<0.05). Conclusion: The results indicated TMJ condylar chondrocytes has a certain adaptability to static compression.
Key words:
condylar chondrocytes,
static pressure,
MMP-13
中图分类号:
徐高丽，徐婷，汪淑华，柳毅，阎帆，谷志远. 不同静压力下髁突软骨细胞MMP-13的表达变化研究[J]. 《口腔颌面外科杂志》, 2016, 26(3): 156-161.
XU Gao-li, XU Ting, WANG Shu-hua, LIU Yi, YAN Fan, GU Zhi-yuan. The Effect of the Static Pressure on Expression of MMP-13 in Condylar Chondrocytes[J]. 《Journal of Oral and Maxillofacial Surgery》, 2016, 26(3): 156-161.