骨髓间充质干细胞联合富血小板纤维蛋白治疗小鼠颞下颌关节骨关节炎的研究

doi:10.12439/kqhm.1005-4979.2026.01.003

摘要/Abstract

关键词: 颞下颌关节, 骨关节炎, 骨髓间充质干细胞, 富血小板纤维蛋白

Key words: temporomandibular joint, osteoarthritis, bone marrow mesenchymal stem cells, platelet-rich fibrin

兰玲莉, 谷巍, 王雪峰, 韩尚志, 陈喜波. 骨髓间充质干细胞联合富血小板纤维蛋白治疗小鼠颞下颌关节骨关节炎的研究[J]. 《口腔颌面外科杂志》, 2026, 36(1): 19-26.

LAN Lingli, GU Wei, WANG Xuefeng, HAN Shangzhi, CHEN Xibo. Effect of bone marrow mesenchymal stem cells combined with platelet-rich fibrin on treatment of temporomandibular joint osteoarthritis in mice[J]. 《Journal of Oral and Maxillofacial Surgery》, 2026, 36(1): 19-26.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2026/V36/I1/19

图/表 7

表1 RT-qPCR引物序列

Table 1 RT-qPCR primer sequences

基因	引物序列(5'-3')	引物序列(3'-5')
β-actin	CGCGAGTACAACCTTCTTGC	CGTCATCCATGGCGAACTGG
Aggrecan	GACCTGTGTGAGATCGACCA	GGTCGGGAAAGTGGCGATAA
Sox9	CAGACCAGTACCCGCATCTG	GCTCTCGTTCAGCAGTCTCC
Col1a1	CTTTGTGGACCTCCGGCTC	AGCTGACTTCAGGGATGTCTTC
Col2a1	CTGTGAAGACCCAGACTGCC	TTCTCCTTTCTGCCCCTTTGG

图1 BMSCs干细胞表面标志物的流式细胞术检测结果 A—E.依次为BMSCs干细胞表面标志物CD29、CD44、CD90、CD105及CD166检测结果。

Figure 1 The flow cytometry of stem cell surface markers in BMSCs

图2 BMSCs多向诱导分化结果 A. BMSCs成骨诱导后茜素红染色图（×100）；B. BMSCs成脂诱导后油红O染色图（×100）；C. BMSCs成软骨诱导后阿利新蓝染色图(×200)。

Figure 2 The multi-lineage differentiation results of BMSCs

图3 各组髁突软骨HE染色图片（×200） A—D.术后1周时，各组髁突软骨HE染色图；E—H.术后2周时，各组髁突软骨HE染色图；I—L.术后4周时，各组髁突软骨HE染色图；M—P.术后8周时，各组髁突软骨HE染色图。①表示纤维层；②表示增殖层；③表示肥大软骨层；④表示钙化软骨层。黑色箭头示纤维层增厚；红色箭头示细胞减少；黄色箭头示软骨细胞团。

Figure 3 HE staining of the condyle cartilages in each group (×200)

图4 各组番红O染色图片（×100） A—D.依次为术后1周时，各组髁突软骨番红O染色图；E—H.依次为术后2周时，各组髁突软骨番红O染色图；I—L.依次为术后4周时，各组髁突软骨番红O染色图；M—P.依次为术后8周时，各组髁突软骨番红O染色图。

Figure 4 Safranin O staining of the condyle cartilages in each group (×100)

表2 各组不同时间点改良Mankin评分结果

Table 2 Modified Mankin scores in each group at different points

组别	术后1周	术后2周	术后4周	术后8周
对照组	1.88±0.64	5.38±0.74	6.63±0.92	8.38±0.92
BMSCs组	1.63±0.52	4.00±0.76^①	5.25±1.04^①	6.88±0.64^①
PRF组	0.88±0.64^①②	3.71±0.71^①	6.00±0.93	8.00±0.76
BMSCs+PRF组	0.38±0.52^①②	3.25±0.71^①②	5.38±0.92^①	4.38±0.74^①②③

图5 术后8周各组软骨相关基因的mRNA相对表达量 A.术后8周各组Aggrecan mRNA相对表达量；B.术后8周各组Col1a1 mRNA相对表达量；C.术后8周各组Col2a1 mRNA相对表达量；D.术后8周各组Sox9 mRNA相对表达量。*表示P<0.05，***表示P<0.001，与对照组比较；##表示P<0.01，###表示P<0.001，与BMSCs组比较；&&&表示P<0.001，与PRF组比较。

Figure 5 Relative mRNA expression levels of cartilage-related genes in each group at 8 weeks postoperatively

参考文献 22

[1]	Badel T, Zadravec D, Bašić Kes V, et al. Orofacial pain - diagnostic and therapeutic challenges[J]. Acta Clin Croat, 2019, 58(Suppl 1): 82-89.
[2]	钟阳, 满城. 颞下颌关节骨关节炎发病机制的研究进展[J]. 口腔颌面外科杂志, 2021, 31(4): 245-248. URL
[3]	邢丹, 陈耀龙, 曾宪涛, 等. 2019年《OARSI非手术治疗膝、髋及多关节骨关节炎临床实践指南》方法学解读和推荐意见阐释[J]. 中国循证医学杂志, 2020, 20(3): 258-266.
[4]	Murphy JM, Fink DJ, Hunziker EB, et al. Stem cell therapy in a caprine model of osteoarthritis[J]. Arthritis Rheum, 2003, 48(12): 3464-3474.
[5]	Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: technological concepts and evolution[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 101(3): e37-e44.
[6]	王拓, 杨琴秋, 董露, 等. 富血小板纤维蛋白诱导口腔缺损软组织的修复与再生[J]. 中国组织工程研究, 2016, 20(7): 957-965.
[7]	Damsaz M, Castagnoli CZ, Eshghpour M, et al. Evidence-based clinical efficacy of leukocyte and platelet-rich fibrin in maxillary sinus floor lift, graft and surgical augmentation procedures[J]. Front Surg, 2020, 7: 537138.
[8]	Fang DD, Long ZB, Hou J. Clinical application of concentrated growth factor fibrin combined with bone repair materials in jaw defects[J]. J Oral Maxillofac Surg, 2020, 78(6): 882-892.
[9]	Albilia DMD M, Herrera-Vizcaíno DDS C, Weisleder BSc H, et al. Liquid platelet-rich fibrin injections as a treatment adjunct for painful temporomandibular joints: Preliminary results[J]. CRANIO^®, 2020, 38(5): 292-304.
[10]	Khojasteh A, Hosseinpour S, Nazeman P, et al. The effect of a platelet-rich fibrin conduit on neurosensory recovery following inferior alveolar nerve lateralization: A preliminary clinical study[J]. Int J Oral Maxillofac Surg, 2016, 45(10): 1303-1308.
[11]	Xiong SH, Qiu LH, Zhao JH, et al. The role of platelet concentrates in facial fat grafting[J]. Ann Plast Surg, 2018, 81(6S Suppl 1): S117-S123.
[12]	Zhang YD, Ruan Z, Shen MH, et al. Clinical effect of platelet-rich fibrin on the preservation of the alveolar ridge following tooth extraction[J]. Exp Ther Med, 2018, 15(3): 2277-2286.
[13]	Wang ZS, Feng ZH, Wu GF, et al. The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering[J]. Sci Rep, 2016, 6: 28126.
[14]	Xu L, Polur I, Lim C, et al. Early-onset osteoarthritis of mouse temporomandibular joint induced by partial discectomy[J]. Osteoarthritis Cartilage, 2009, 17(7): 917-922.
[15]	Lan LL, Jiang YM, Zhang WN, et al. Expression of Notch signaling pathway during osteoarthritis in the temporomandibular joint[J]. J Craniomaxillofac Surg, 2017, 45(8): 1338-1348.
[16]	McCarthy HE, Bara JJ, Brakspear K, et al. The comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse[J]. Vet J, 2012, 192(3): 345-351.
[17]	郭泰, 宣雯霞, 栗树伟, 等. 胰岛素样生长因子治疗兔早期膝关节损伤的实验研究[J]. 临床医药实践, 2016, 25(11): 832-835.
[18]	李雪燕, 肖迪, 陈瞰, 等. 骨关节炎下颞下颌关节注射Nell-1对关节软骨保护作用的研究[J]. 现代口腔医学杂志, 2017, 31(1): 1-5.
[19]	王鹏, 陈瞰, 任笑春, 等. 软骨分化间充质干细胞对颞下颌关节炎软骨修复的影响[J]. 临床口腔医学杂志, 2017, 33(4): 203-207.
[20]	杨世茂, 王明国, 李静, 等. 富血小板纤维蛋白与富血小板血浆体外释放生长因子的比较及其对脂肪干细胞增殖分化的影响[J]. 华西口腔医学杂志, 2012, 30(6): 641-644, 649.
[21]	雍志军, 贾帅军, 韩林章, 等. 富血小板纤维蛋白对兔骨髓间充质干细胞成软骨分化的影响[J]. 现代生物医学进展, 2014, 14(8): 1407-1410.
[22]	高玉宇. 富血小板浓缩物对兔髁突软骨细胞生物学特性的体外研究[D]. 昆明: 昆明医科大学, 2019.

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