FGF-9 Regulates Osteogenic Differentiation of Mouse Maxillary Primordium Mesenchymal Cells

doi:10.3969/j.issn.1005-4979.2014.06.003

Abstract

Abstract: Objective: To investigate the regulatory effect of FGF-9 for the osteogenic differentiation of mouse maxillary primordium mesenchymal cells (MPMCs) cultured in vitro. Methods: MPMCs were obtained from the E12.5 embryos and cultured in vitro. Cells of the primary passage were cultured in the osteogenic medium for 1 week, and 10 nmol/L FGF-9 human recombinant protein was added in the medium in experimental group. qPCR，immunofluorescence staining and Alizarin red staining were used to evaluate the osteogenic capability of MPMCs. Results: E12.5 MPMCs expressed positive FGF-9 and FGFR-3 protein in vitro. Osteogenic induction could stimulate the expression of osteogenic markers, including ALP, Runx2 and OCN. However, exogenous FGF-9 could inhibit the expression of ALP, Runx2 and OCN, and reduced the formation of calcium nodules simultaneously in MPMCs. Conclusion: FGF-9 can negatively regulate the osteogenic differentiation of MPMCs in vitro.

Key words: fibroblast growth favtor 9（FGF-9）, maxillary primordium mesenchymal cells, osteogenic differentiation, rats

摘要： 目的：研究成纤维细胞生长因子9（FGF-9）对体外培养的小鼠上颌突间充质细胞成骨分化的调控作用。方法：体外培养E12.5（胚胎第12.5天）的小鼠上颌突间充质细胞，取第1代细胞进行成骨诱导，实验组加入FGF-9人重组蛋白，诱导培养1周后通过qPCR、免疫荧光和茜素红染色检测其成骨能力。结果：体外培养的E12.5小鼠上颌突间充质细胞表达FGF9和FGFR3。成骨诱导可促进上颌突间充质细胞表达成骨标记物ALP、Runx2和OCN；加入FGF-9人重组蛋白可降低成骨标记物ALP、Runx2和OCN的表达，减少钙结节形成。结论：体外培养时，FGF9参与负调控上颌突间充质细胞的成骨分化。

关键词: 成纤维细胞生长因子9（FGF-9）, 上颌突间充质细胞, 成骨分化, 鼠

CLC Number:

LI Jin-chao, LIU Ji-guang, LIU Zhuo, GAO Zi-long, JIANG Xin, LI Shou-hong. FGF-9 Regulates Osteogenic Differentiation of Mouse Maxillary Primordium Mesenchymal Cells[J]. 《Journal of Oral and Maxillofacial Surgery》, 2014, 24(6): 413-.

李金超，刘继光，刘茁，高子龙，江欣，李守宏. FGF-9参与调控小鼠上颌突间充质细胞体外成骨分化的研究[J]. 《口腔颌面外科杂志》, 2014, 24(6): 413-.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2014.06.003

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2014/V24/I6/413

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