Abstract:

Objective: To investigate whether the gene expression changes of the primary cilia and osteoblast in the alveolar bone are associated with occlusal hypofunction. Methods: The unloading model was established in the right mandible on 6-week-old wild type (WT) male mice and the left side was used as a self-control study. Samples were sacrificed 2 weeks after the operation, and the parameters of alveolar bone were scanned and analyzed by micro-CT. The changes in the number and position of osteoblasts and osteoclasts were observed histologically, and real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of osteoblastogenesis and osteoclastogenesis. The changes in the number and location of primary cilia were observed by co-staining, and the expression of cilium-related genes was detected. The Pearson correlation analysis was used to detect the expression of cilium-related genes and osteoblastogenesis, and the colocalization was observed by co-staining.Results: Analysis of the unloading side revealed a significant decrease in bone volume/total volume (BV/TV) (P<0.05). The number of osteoblasts decreased while the number of osteoclasts increased significantly (P<0.01). Both the occurrence rate of primary cilia and expression of cilium-related genes showed a decreasing trend (P<0.05). Furthermore, the expression of osteoblastogenesis and intraflagellar transport genes were significantly correlated (P<0.05). Co-staining results showed that osteoblasts were located on the top of root furcation, and there were primary cilia on the surface.Conclusion: The parameters of alveolar bone on the experimental side were significantly decreased and bone resorption accelerated on the unloading side. Both the number of osteoblasts and primary cilia as well as the expression of related genes decreased significantly and the expression levels of related genes were positively correlated.

Key words: occlusal hypofunction, osteoblast, primary cilia, alveolar bone remolding

摘要：

目的： 探究咬合力缺失牙槽骨中成骨基因与初级纤毛基因表达变化的相关性。方法： 建立6周龄雄性野生型（wild type， WT）小鼠右侧下颌咬合力缺失模型，将左侧作为自身对照。2周后收样，应用微型CT（micro-CT）扫描分析骨改建情况。用组织学染色法观察骨组织内细胞改变情况，实时定量聚合酶链反应（real-time quantitative polymerase chain reaction， RT-qPCR） 法检测成骨及破骨基因表达。用荧光染色法观察初级纤毛改变情况，并检测纤毛相关基因表达。对成骨与纤毛相关基因表达水平行Pearson相关性分析，用荧光染色法观察二者分布情况。方法： 小鼠下颌失力侧骨体积分数（bone volume/total volume， BV/TV）显著下降（P<0.05）。成骨细胞减少伴基因表达量降低，破骨细胞反之（P<0.01）；纤毛出现率和基因表达显著下降（P<0.05）。小鼠失力侧的成骨与纤毛相关基因表达呈正相关关系（P<0.05）；成骨细胞位于根分叉顶部，且表面有初级纤毛分布。结果： 咬合力缺失后，牙槽骨密度降低、骨吸收加快，成骨细胞和纤毛数量均显著减少，相关基因表达下降，且二者呈正相关。

关键词: 咬合力缺失, 成骨细胞, 初级纤毛, 牙槽骨改建

CLC Number: 

• R782