摘要： 目的： 将骨膜、骨髓单个核细胞（BM-MNCs）、纳米羟基磷灰石(纳米?鄄HA)复合应用于引导性骨再生，为临床修复骨缺损探索一条有效的途径。方法：  6~8 月龄实验用白兔 18 只，在兔双侧下颌骨各制备一10 mm×8 mm，深5 mm的骨缺损，按随机数字表法分为 3 组，每组6只，即A组用自体游离骨膜、骨髓单个核细胞和纳米羟基磷灰石复合修复，B组用自体游离骨膜和纳米羟基磷灰石修复，C组单纯用纳米羟基磷灰石充填修复。分别于术后第4、 8 、12 周末用空气栓塞法处死动物，每次每组处死2只，标本行肉眼、X线、扫描电镜及组织学观察,计算缺损区新骨面积。 结果： 实验组A组在术后 4、8、12 周的骨密度值均高于其他两组（P<0.05）。环境扫描电镜观察显示， A组新生骨和原有骨结合致密且骨结构相似。组织学观察表明，A组材料大部分降解,大量成熟骨形成,较少材料包裹于其中，各项指标均优于其他组。结论：骨膜、骨髓单个核细胞、纳米羟基磷灰石复合的成骨作用优秀，可有效促进骨缺损愈合。

关键词: 骨膜；骨髓单个核细胞 ;引导性骨再生； 骨缺损, 兔

Abstract: Objective: The aim of this study was to identify the effects of a compound with free bone periosteum- bone marrow mononuclear cells (BM-MNCs), and nano-HA in guided bone regeneration (GBR).  Methods: Eighteen white rabbits were randomly and equally divided into 3 groups. In each rabbit, a 10 mm×8 mm bone defect was created in both sides of the mandible. In group A, bone defects were restored with  free periosteum-bone, BM-MNCs, HA; in group B, defects were repaired with free periosteum-bone, HA; in group C, only nano-HA were applied. 2 rabbits in each group were sacrificed at 4, 8 and 12 weeks after operation. Specimen were examined by gross examination, radiographic examination, scanning electron microscopy, and histology observation. Results: Comparison between group A and group B, C, the significant difference of bone mineral density values (P<0.05) manifested at 4, 8 and 12 weeks after restoration. Under  electron microscopy, the newly formed bone showed close integration and structural similarities with the surrounding bone. Histological observation showed that most restoration materials in group A was degraded, a large number of mature bone formation, less material wrapped in which indicated a better result than other groups. Conclusion: The free bone periosteum-bone marrow mononuclear cells cement HA has a significant osteogenic competence. It can efficiently repair bone defects.

Key words: periosteum-bone；bone marrow mononuclear cells, guided bone regeneration , bone defect；rabbit

中图分类号: 

• R782.4