摘要： 目的：探讨姜黄素对口腔鳞癌细胞系HN13细胞骨架的影响及其相关的分子机制。方法: 用考马斯亮蓝染色分析法检测不同浓度姜黄素（0、10、20、40 μmol/L）作用24 h 的细胞，观察姜黄素对鳞癌细胞骨架的影响。用免疫组织化学法半定量检测不同浓度姜黄素（0、10、20、40 μmol/L）作用24 h后细胞内F-actin含量的变化。用 Western印迹法检测不同浓度姜黄素（0、10、20、40 μmol/L）作用24 h后ROCK2及p-ROCK2蛋白的表达。 结果:在低浓度姜黄素(10 μmol/L)作用24 h后细胞骨架结构收缩、染色变淡、稀疏、变细，排列方式发生变化，但尚存一部分应力纤维；随着姜黄素浓度的增加(20 μmol/L)，细胞骨架变化更加明显，表现为细胞骨架含量明显减少，放射状应力纤维几乎完全消失；当姜黄素浓度为40 μmol/L时，细胞骨架轮廓已不清晰，染色更淡，减少更加明显，并有断裂，多数细胞变形明显。不同浓度姜黄素干预细胞24 h 后, 细胞F-actin含量减少，各组间差异有统计学意义（P<0.01）。 Western印迹法检测结果显示不同浓度姜黄素（0、10、20、40 μmol/L）作用24 h后细胞内ROCK2及p-ROCK2蛋白的表达显著下调。结论:姜黄素可诱导鳞癌细胞骨架改变，其作用机制与抑制Rho/ROCK信号通路的效应分子ROCK2及p-ROCK2活化有关。

关键词: Rho/ROCK信号通路, 姜黄素, 鳞癌, 细胞骨架

Abstract: Objective: The present article aimed to study whether the effects of curcumin are related to  cytoskeleton variation in OSCC cell line HN13 and its mechanism. Methods: HN13 cells were treated and exposed with different concentrations of curcumin（0，10，20，40 μmol/L）. After 24 h, Coomassie blue staining was used to measure the cystoskeleton o f HN13 cells. Immunohistochemistry was used to observe the expression of F-actin. The expression of ROCK2 and phosphated ROCK2 were detected by Western blot. Results: Qualitative morphological evaluation revealed that  HN13 cell exposed to curcumin with different concentration became  obvious deformation. The cytoskeletal structures shrinked, the staining became faded, stress fibers reduced. Immunohistochemistry revealed the staining of F-actin appeared somewhat weaker in HN13 cells which exposed in high concentration of curcumin. When treated for 24 h, the expression of ROCK2 and p-ROCK2 in HN13 cells was decreased. Conclusion: Curcumin may trigger alterations of the cystoskeleton of HN13 cell line by inhibiting activity of Rho/ROCK2 pathway.

Key words: Rho/ROCK2 pathway, curcumin, oral squamous cell carcinoma; , cystoskeleton

中图分类号: 

• R739.86