三七总皂苷对兔BMSCs的成骨分化及TGF-&beta;1表达的影响

doi:10.3969/j.issn.1005-4979.2014.04.003

《口腔颌面外科杂志》 ›› 2014, Vol. 24 ›› Issue (4): 261-. doi: 10.3969/j.issn.1005-4979.2014.04.003

三七总皂苷对兔BMSCs的成骨分化及TGF-β1表达的影响

唐弈遥1，周诺2，郭宇航1，汪延1，林海运1

1. 广西医科大学口腔医学院，广西南宁 530021；2. 广西医科大学附属口腔医院口腔颌面外科，广西南宁 530021

出版日期:2014-08-28 发布日期:2021-11-04
通讯作者: 周诺，主任医师. E-mail:nuozhou@hotmail.com
作者简介:唐弈遥（1988—），男，广西岑溪人，硕士研究生. E-mail: tommyistommy29@gmail.com
基金资助:
国家自然科学基金资助项目（81160132）；广西自然科学基金（0991013）

Panax Notoginseng Saponins Mediate Rabbit Bone Marrow Mesenchymal Stem Cells Osteodifferentiation

TANG Yi-yao1, ZHOU Nuo2, GUO Yu-hang1, WANG Yan1, LIN Hai-yun1

1. School of Stomatology, Guangxi Medical University, Nanning 530021; 2. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Nanning 530021, Guangxi Province, China

Online:2014-08-28 Published:2021-11-04

摘要/Abstract

摘要： 目的：观察三七总皂苷（panax notoginseng saponins，PNS）对兔骨髓间充质干细胞(bone marrow stromal cells，BMSCs)成骨分化及TGF-β1在基因水平表达变化的影响。方法：体外分离培养兔BMSCs，观察不同浓度PNS（50、100、200 mg/L）对兔BMSCs的影响，采用MTT法检测细胞增殖，茜素红染色观察钙结节形成，qRT-PCR检测TGF-β1表达水平。结果：各浓度PNS培养下的细胞增殖无明显差异（P＞0.05）；100、200 mg/L PNS组的钙结节数量和TGF-β1的表达水平均高于阴性组（P＜0.05）。结论：PNS可促进兔BMSCs的成骨分化并刺激其分泌TGF-β1，但无明显促细胞增殖作用。

关键词: 三七总皂苷； , 成骨分化； , 骨髓间充质干细胞； , 转化生长因子

Abstract: Objective: To observe the osteoinduction effect of panax notoginseng saponins (PNS) on rabbit bone marrow mesenchymal stem cells (BMMSCs), and the expression level change of TGF-β1. Methods: Rabbit BMMSCs were isolated and cultured in vitro. To observe the influence of different concentration of PNS (50、100、200 mg/L) on rabbit BMMSCs, several methods were performed, including cell culture technique, MTT colorimetric assay, Dahl McGec Russell's Alizarin red staining for calcium and qRT-PCR explore TGF-β1 expression level. Results: There was no significant difference of proliferation capacity of BMMSCs between various concentration of PNS(P＞0.05). The calcium nodule and TGF-β1 expression level in 100、200 mg/L PNS groups were significantly higher than negative group (P＜0.05). Conclusion: PNS can promote the osteogenic differentiation and secretion of TGF-β1 of rabbit BMMSCs, but PNS is not effective enough for mediation of BMMSCs proliferation.

Key words: panax notoginseng saponins, osteogenic differentiation, bone mesenchymal stem cells, transforming growth factor

中图分类号:

唐弈遥1，周诺2，郭宇航1，汪延1，林海运1. 三七总皂苷对兔BMSCs的成骨分化及TGF-β1表达的影响[J]. 《口腔颌面外科杂志》, 2014, 24(4): 261-.

TANG Yi-yao1, ZHOU Nuo2, GUO Yu-hang1, WANG Yan1, LIN Hai-yun1. Panax Notoginseng Saponins Mediate Rabbit Bone Marrow Mesenchymal Stem Cells Osteodifferentiation[J]. 《Journal of Oral and Maxillofacial Surgery》, 2014, 24(4): 261-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2014/V24/I4/261

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