摘要： 目的：探讨miRNA-31这一微小RNA对糖尿病小鼠来源骨髓间充质干细胞（BMSCs）体外成骨分化的影响。方法：采用高糖高脂饮食加链脲佐菌素腹腔注射诱导建立Ⅱ型糖尿病小鼠模型，造模8周后，在体外原代分离培养BMSCs，检测其miRNA-31和成骨分化情况，并与正常对照组比较。再将糖尿病小鼠来源的BMSCs体外分别转染miRNA-31模拟物和miRNA-31 抑制剂，再检测比较2种BMSCs的体外成骨分化能力。结果：糖尿病小鼠造模成功8周后，micro CT检测结果显示与正常对照组小鼠相比，模型组小鼠明显骨密度降低，呈现典型骨质疏松。将糖尿病小鼠来源BMSCs体外培养后，发现与对照组相比，其成骨分化能力降低，miRNA-31表达升高。而转染miRNA-31抑制剂后可部分解除糖尿病微环境对BMSCs成骨分化的抑制。结论：糖尿病微环境下BMSCs高表达miRNA-31，并可抑制BMSCs的成骨分化。

关键词: 糖尿病性骨质疏松症；  , 骨髓间充质干细胞；  , 成骨分化；  , microRNA

Abstract: Objective: To investigate the effect of miRNA-31 on the osteogenic differentiation of BMSCs in high-glucose microenvironment. Methods: Diabetic osteoporosis（DOP） mice model was established by high glucose and high fat diet plus STZ intraperitoneal injection. Bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured in vitro. The expression of miRNA-31 were detected in vitro. The osteogenic differentiation ability was detected after miRNA-31 mimics and miRNA-31 inhibitors were transfected respectively. Results: DOP model was successfully established. The results of micro-CT showed that DOP mice had lower bone mineral density. BMSCs isolated from DOP model showed lower osteogenic differentiation ability and higher expression of miRNA-31. After transfection of miRNA-31 inhibitors, the osteogenic differentiation ability of BMSCs could be partially restored. Conclusions: BMSCs in hyperglycemia microenvironment will high express miRNA-31, which may inhibit osteogenic differentiation of BMSCs.

Key words: diabetic osteoporosis, BMSCs, osteogenic differentiation, microRNA

中图分类号: 

• R782.4