淫羊藿苷对犬骨髓间充质干细胞增殖、成骨和成脂分化的影响

doi:10.3969/j.issn.1005-4979.2014.04.006

《口腔颌面外科杂志》 ›› 2014, Vol. 24 ›› Issue (4): 276-. doi: 10.3969/j.issn.1005-4979.2014.04.006

淫羊藿苷对犬骨髓间充质干细胞增殖、成骨和成脂分化的影响

刘天麟1,2，张学斌1,3，张忻1，潘汀2，吴艳玲2，黄远亮2

1. 同济大学口腔医学院口腔生物医学及转化医学实验室，上海 200072；2. 同济大学附属上海市东方医院口腔科，上海 200120；3. 烟台市口腔医院口腔种植科，山东烟台 264008

出版日期:2014-08-28 发布日期:2021-11-04
通讯作者: 黄远亮，教授，主任医师. E-mail:13301921076@163.com
作者简介:刘天麟(1980—)，男，安徽人，博士研究生，主治医师. E-mail: 525ya@163.com
基金资助:
国家自然科学基金资助项目（81070806）；上海市东方医院东方朝阳人才计划项目（DFZY-09）

Effect of Icariin on the Proliferation,Osteogenic Differentiation, and Adipogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells

LIU Tian-lin1,2, ZHANG Xue-bin1, ZHANG Xin1, PAN Ting2, WU Yan-ling2, HUANG Yuan-liang2

1. Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai 200072; 2. Department of Stomatology, Oriental Hospital, Tongji University, Shanghai 200120; 3. Department of Dental Implant, Yantai Hospital of Stomatology, Yantai 264008, Shandong Province, China

Online:2014-08-28 Published:2021-11-04

摘要/Abstract

摘要： 目的：研究淫羊藿苷对犬骨髓间充质干细胞（bone marrow mesenchymal stem cells ，BMSCs)增殖和成骨、成脂分化的影响。方法：将淫羊藿苷配制成终浓度为1×10-9、1×10-8、1×10-7、1×10-6、1×10-5、1×10-4 mol/L的溶液，以0 mol/L组为空白对照组，分别用于体外培养犬BMSCs。CCK-8试剂盒检测细胞增殖情况，碱性磷酸酶试剂盒检测碱性磷酸酶活性。然后实验分为淫羊藿苷组和对照组，分别在含或不含成骨、成脂诱导液条件下，行成骨、成脂诱导分化实验，通过茜素红和油红O染色检测各组钙沉积和脂质形成面积。结果：发现淫羊藿苷1×10-6 mol/L组促进犬BMSCs增殖，而1×10-4 mol/L组抑制增殖。碱性磷酸酶活性与淫羊藿苷浓度呈剂量依赖性关系。不含成骨、成脂诱导液条件下，各组均几乎未见明显钙沉积和脂质形成；含成骨、成脂诱导液条件下，淫羊藿苷组（1×10-6 mol/L）钙沉积面积高于对照组，脂质形成面积低于对照组。结论：淫羊藿苷能促进犬BMSCs增殖和成骨分化，并抑制其成脂分化。

关键词: 淫羊藿苷； , 骨髓间充质干细胞； , 成骨分化； , 成脂分化； , 犬

Abstract: Objective: To investigate the effects of icariin on the proliferation and osteogenic / adipogenic differentiation of canine bone marrow mesenchymal stem cells (BMSCs). Methods: Canine BMSCs were treated and cultured with icariin in concentrations of 1×10-9, 1×10-8, 1×10-7, 1×10-6, 1×10-5, 1×10-4 mol/L for experiment, and 0 mol/L was used as a blank control. To compare the in vitro osteogenic / adipogenic differentiation ability of BMSCs incubated in osteogenic / adipogenic medium, we performed a CCK-8 assay for cell proliferation, an alkaline phosphatase (ALP) activity assay, a calcium deposition assay (alizarin red staining, ARS), and a lipids deposition assay (oil red O staining). Results: Icariin promoted proliferation of canine BMSCs in 1×10-6 mol/L concentration, and 1×10-4 mol/L concentration icariin inhibited proliferation. The relation between canine BMSCs ALP activity and icariin concentration demonstrated in a dose-dependent manner. After culture in nonosteogenic / adipogenic medium, there were no significant difference between calcium deposition and lipids deposition in all groups. But after culture in osteogenic / adipogenic medium, proportion of calcium deposition in Icariin group（1×10-6mol/L） was higher than control group. On the contrary, proportion of lipid in icariin group（1×10-6mol/L） was lower than the control group. Conclusion: Icariin can promote the proliferation and osteogenic differentiation of canine BMSCs and inhibit adipogenic differentiation.

Key words: icariin; , BMSCs; , osteogenic differentiation; , adipogenic differentiation

中图分类号:

刘天麟1,2，张学斌1,3，张忻1，潘汀2，吴艳玲2，黄远亮2. 淫羊藿苷对犬骨髓间充质干细胞增殖、成骨和成脂分化的影响[J]. 《口腔颌面外科杂志》, 2014, 24(4): 276-.

LIU Tian-lin1,2, ZHANG Xue-bin1, ZHANG Xin1, PAN Ting2, WU Yan-ling2, HUANG Yuan-liang2. Effect of Icariin on the Proliferation,Osteogenic Differentiation, and Adipogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells[J]. 《Journal of Oral and Maxillofacial Surgery》, 2014, 24(4): 276-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2014/V24/I4/276

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淫羊藿苷对犬骨髓间充质干细胞增殖、成骨和成脂分化的影响

Effect of Icariin on the Proliferation,Osteogenic Differentiation, and Adipogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells

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淫羊藿苷对犬骨髓间充质干细胞增殖、成骨和成脂分化的影响

Effect of Icariin on the Proliferation,Osteogenic Differentiation, and Adipogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells

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