摘要： 目的：体外模拟糖尿病病人高血糖状态，观察高糖对人骨髓间充质干细胞（human mesenchymal stem cells, hMSCs）成骨能力的影响。方法：将使用不同含糖量培养液体外培养的hMSCs分为5.5mmol/L生理糖浓度组、25 mmol/L高糖组、44 mmol/L高糖组。CCK?鄄8试剂盒检测各组hMSCs的增殖速度；骨诱导7、14、21 d时分别观察高糖对hMSCs骨向分化相关基因骨钙素(OCN)、骨桥蛋白（OPN）、Runx相关因子2(Runx?鄄2)的表达，碱性磷酸酶（ALP）活性，钙沉积量的影响。结果：两个高糖组相对生理糖组，有抑制hMSCs的增殖能力（P<0.05），且抑制ALP活性及细胞外钙基质沉积，下调了骨向分化相关基因OCN、OPN、Runx?鄄2表达水平（P<0.01），该抑制作用随糖的浓度升高而加强。结论：高糖显著抑制了hMSCs生物矿化过程，并以浓度依赖的方式降低hMSCs的增殖及成骨能力。

关键词: 高血糖；  , 人骨髓间充质干细胞；  , 成骨分化；  , 体外研究

Abstract: Objective: The current study designed to examine whether  human bone mesenchymal stem cells（hMSCs）modulate early osteogenic metabolism during exposure to hyperglycemia condition in vitro. Methods： Third-passage hMSCs were used for experiments and divided into 3 groups: 5.5 mmol/L normal glucose control group，25 mmol/L high glucose group, and 44 mmol/L high glucose group. Cell proliferation was measured by counting kit (CCK)-8. The effects of high glucose on hMSCs osteogenesis related gene OCN、OPN and Runx2 mRNA expression，ALP  activity，calcium deposits were measured at days 7, 14, and 21. Results: In higher glucose concentration (25 and 44 mmol/L) groups, hMSCs proliferation, ALP activity, calcium deposition, OCN、OPN、Runx2 mRNA expression were significantly reduced as compared with 5.5 mmol/L normal glucose group. High glucose showed an inhibition effect on activities of hMSCs in a concentration-dependent manner. Conclusion: High glucose inhibits biomineralization process in hMSCs; High glucose may inhibit the proliferation and differentiation of hMSCs in a concentration-dependent manner.

Key words: high glucose, human bone mesenchymal stem cells, osteogenic differentiation； in vitro

中图分类号: 

• R392.2