摘要： 目的：通过动物实验检测碳酸化羟基磷灰石支架材料（carbonated hydroxyapatite，CAP）的骨传导性和生物吸收性。并探讨骨形成蛋白-2（rhBMP-2）、重组人巨噬细胞集落刺激因子（rhM-CSF）对CAP成骨特性和生物吸收的影响。方法：选取45只雄性SD大鼠，制备双侧胫骨临界性骨缺损模型，以复合骨形成蛋白-2（rhBMP-2）的碳酸化羟基磷灰石支架材料、复合重组人巨噬细胞集落刺激因子（rhM-CSF）的碳酸化羟基磷灰石支架材料、单纯的碳酸化羟基磷灰石支架材料作为实验组，羟基磷灰石（hydroxyapatite，HAP）作为对照组，植入大鼠胫骨缺损处，并设立空白对照组。术后2、4和8周，通过组织学观察对比新骨形成和材料吸收降解情况。结果：实验组和对照组材料均能完全充填骨缺损，材料界面与骨组织结合紧密，显示了良好的生物相容性和成骨性能。随着植入时间的延长，实验组材料可逐渐降解并被新生骨爬行替代，而对照组未见显著降解和新生骨替代。rhM-CSF能够促进碳酸化羟基磷灰石材料的降解，与CAP组、CAP/rhBMP-2复合物组比较，差异有统计学意义。结论：CAP具有出色的骨传导性和生物吸收性，是一种良好的骨再生移植物。且该支架材料的成骨性和生物降解能够被成骨及破骨细胞因子所调控。

关键词: 碳酸化羟基磷灰石支架；  , 骨形成蛋白-2（BMP-2）；  , 巨噬细胞集落刺激因子（M-CSF）；  , 骨缺损重建；  , 鼠

Abstract: Objective:  ① To evaluate the osteoconductivity and bioresorbability of the carbonated hydroxyapatite (CAP) scaffold. ② To evaluate the effects of rhBMP-2 and rhM-CSF on bone defect reconstruction with CAP scaffold.  Methods: 45 male SD rats were comprised in this experiment and randomly divided into 5 groups. A 4 mm×4 mm critical-size bone defect was created in both sides of tibia in all rats. Group A, CAP was placed into the defect; Group B, placed with CAP/rhBMP-2; Group C, CAP/rhM-CSF was placed; Group D, HAP was placed; and Group E, bone defect left void as control. At 2- week, 4- week and 8-week after restoration, the formation of new bone and the absorption of the materials were observed under histological examination. Results: Bone defects were filled completely in experimental groups. The biocompatiblility was fine. Bone directly bonded to the CAP scaffold. During the native bone remodeling process, the CAP scaffold was replaced by new bone as CAP degraded in the experimental groups. But in the HAP group, the decomposition and replacing processes was not significant. rhM-CSF promoted significantly the degradation of carbonated hydroxyapatite scaffold since week 4. Conclusion:  ①Carbonated hydroxyapatite scaffold showed good osteoconductivity and bioresorbabiloly in vivo. ② rhM-CSF can promote the degradation of carbonated hydroxyapatite scaffold.

Key words: carbonated hydroxyapatite scaffold, BMP-2, M-CSF, bone defect reconstruction, rats

中图分类号: 

• R782.2