小鼠上下颌骨发育差异性表达基因的生物信息学分析

doi:10.3969/j.issn.1005-4979.2014.06.004

摘要/Abstract

摘要： 目的：上、下颌骨的发育差异可能是由于两者差异性地表达某些基因而造成的。本文拟对E9.5（胚胎第9.5天）小鼠上、下颌弓差异性表达的基因进行系统的生物信息学分析。方法：从GEO 数据库获取E9.5小鼠上、下颌弓差异性表达的基因，应用DAVID和GeneMANIA数据库分析这些基因之间的相互关系。结果：经过DAVID数据分析，这些在E9.5小鼠上、下颌弓差异性表达的基因被富集到不同的生物学过程或分子功能的子集中，如“转录因子活性”、“系统发育”和“骨骼系统发育”等。其中BTB7B、SOX10、TSHZ2、GSC、GLIS2、MEIS1、CITED2、IRF9、BARX1、MSX1、DLX6、CSRNP1、HEYL、MKX、PITX1和NFIB，这16个基因被富集到“转录因子活性”这一分子功能子集。通过GeneMANIA数据库分析，建立了这16个基因及一些预测基因的分子网络图，显示这些基因存在直接或间接的相互作用。结论：上、下颌骨在发育过程中存在许多差异表达的基因，其中对上、下颌骨特异性发育具有重要调控作用的16个转录因子包括BTB7B、SOX10、TSHZ2、GSC、GLIS2、MEIS1、CITED2、IRF9、BARX1、MSX1、DLX6、CSRNP1、HEYL、MKX、PITX1和NFIB，它们彼此密切相关且相互作用形成调控网络。在研究上、下颌骨差异性发育的分子调控机制时，需要关注由这些基因形成的调控网络。

关键词: 上颌骨, 下颌骨, 发育, 基因, 生物信息学分析, 小鼠

Abstract: Objective: The aim of this study was to perform a systematic bioinformatic analysis in differentially expressed genes between the E9.5 mouse maxillary and mandibular arch. Methods: The differentially expressed genes between the E9.5 mouse maxillary and mandibular arch were obtained from the gene expression omnibus database (GEO Datasets). Then the DAVID and GeneMANIA database were used to analyse the relationships during these genes. Results: These differentially expressed genes between the E9.5 mouse maxillary and mandibular arch could be enriched into different "biological process" and "molecular function" subgroups, based on the analysis of DAVID database, including "transcription factor activity", "system development", "skeleton system development", etc. Sixteen genes, including BTB7B, SOX10, TSHZ2, GSC, GLIS2, MEIS1, CITED2, IRF9, BARX1, MSX1, DLX6, CSRNP1, HEYL, MKX, PITX1 and NFIB, were enriched into the "transcription factor activity" subgroup, and these genes were clearly revealed to have direct or indirect interaction with each other based on the analysis of GeneMANIA database. Conclusions: There were many differentially expressed genes during the maxillary and mandibular development process, and sixteen transcription factors, including BTB7B, SOX10, TSHZ2, GSC, GLIS2, MEIS1, CITED2, IRF9, BARX1, MSX1, DLX6, CSRNP1, HEYL, MKX, PITX1 and NFIB, played crucial roles during the differential development of the jaws and closely related to each other. The molecular networks composed by these genes involved in the development of jaws should be paid special attention when study the molecular mechanism of differentially developmental maxilla and mandible.

Key words: maxilla, mandible, development, gene, bioinformatic analysis, rats

中图分类号:

Q343

蒋沂峰，杨希，陈龙. 小鼠上下颌骨发育差异性表达基因的生物信息学分析[J]. 《口腔颌面外科杂志》, 2014, 24(6): 418-.

JIANG Yi-feng, YANG Xi, CHEN Long. Molecular Regulator Network involved in the Development of Mandible and Maxilla: a Bioinformatic Analysis[J]. 《Journal of Oral and Maxillofacial Surgery》, 2014, 24(6): 418-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2014/V24/I6/418

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