放疗早期对口腔颌面部肿瘤患者免疫功能影响的临床研究

doi:10.3969/j.issn.1005-4979.2016.02.007

《口腔颌面外科杂志》 ›› 2016, Vol. 26 ›› Issue (2): 110-114. doi: 10.3969/j.issn.1005-4979.2016.02.007

放疗早期对口腔颌面部肿瘤患者免疫功能影响的临床研究

孔月虹, 涂文勇, 程昌明,丁继平, 徐璇丽

1. 上海交通大学医学院附属第九人民医院口腔颌面头颈肿瘤科，上海市口腔医学重点实验室，上海 200011；
2. 生物芯片上海国家工程研究中心，上海 200436

收稿日期:2015-09-06 修回日期:2015-12-03 出版日期:2016-04-28 发布日期:2016-06-07
通讯作者: 涂文勇，副主任医师. E-mail:tu4you@163.com
作者简介:孔月虹（1988—），女，江苏苏州人，硕士研究生.
基金资助:
CSCO-默克雪兰诺肿瘤研究基金（Y-MT2014-004）

Effects of Radiotherapy on the Immune Function in Patients with Oral and Maxillofacial Cancer

KONG Yue-hong, TU Wen-yong, CHEN Chang-ming, DING Ji-ping, XU Xuan-li

1.Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai Key Laboratory of Stomatology/ Shanghai Research Institute of Stomatology, Shanghai 200011 2.Shanghai Biochip National Engineering Research Center, Shanghai 200436,China

Received:2015-09-06 Revised:2015-12-03 Online:2016-04-28 Published:2016-06-07

摘要/Abstract

摘要： 目的：通过对患者放疗后早期与放疗前的血液，进行免疫细胞检测及外周血基因表达谱芯片检测，分析放疗早期对机体免疫功能的影响。方法：22例口腔颌面肿瘤患者，检测放疗前1周和后1周的血液中，CD3+T、CD4+T、CD8+T、B、NK细胞数量及比例变化。通过表达谱芯片检测筛选放疗前后血液标本的差异基因，用R语言及DAVID数据库行功能分类和信号传导通路相关性分析。结果：患者淋巴细胞及其亚类数目在放疗后明显下降。表达谱芯片筛选出368个免疫炎症相关基因。涉及的信号通路主要是T细胞受体信号通路、黏附分子、NK介导细胞毒效应等相关通路。结论：放疗能部分激活CD8+T及NK细胞介导的杀伤免疫，同时抑制T细胞和NK细胞的分化及其信号传导通路。

关键词: , 放射疗法, , , 口腔肿瘤, , , 免疫功能, , , 表达谱芯片

Abstract: Objective: To analyze the early effects of radiotherapy on the immune function of patients with oral and maxillofacial cancer. Method: Detection of blood immune cells and analyze of peripheral blood gene expression profile were performed. The number and percentage of CD3+T, CD4+T, CD8+T, B and NK cells were examined before and after radiotherapy in 22 patients with oral and maxillofacial cancers. The differential expression gene of blood samples before and after radiotherapy was detected by gene microarray. The gene ontology and signal transduction pathway was analyzed based on R project and DAVID database. Results: The number of lymphocytes and subtypes significantly decreased after radiotherapy. The expression profiles of 368 immune and inflammatory genes were selected. The main signaling pathways involved are the receptor signaling pathway of T cells, the adhesion molecules, and the cytotoxic effect of NK cells. Conclusion: The conventional fractioned radiotherapy can activate cell mediated immunity of CD8+ T and NK cells, but inhibit the differentiation of T cells and NK cells signaling transduction pathway.

Key words: , radiotherapy； oral carcinoma； immune function； peripheral blood transcriptome

中图分类号:

R782
R739.8

孔月虹, 涂文勇, 程昌明,丁继平, 徐璇丽. 放疗早期对口腔颌面部肿瘤患者免疫功能影响的临床研究[J]. 《口腔颌面外科杂志》, 2016, 26(2): 110-114.

KONG Yue-hong, TU Wen-yong, CHEN Chang-ming, DING Ji-ping, XU Xuan-li. Effects of Radiotherapy on the Immune Function in Patients with Oral and Maxillofacial Cancer[J]. 《Journal of Oral and Maxillofacial Surgery》, 2016, 26(2): 110-114.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2016/V26/I2/110

参考文献

[1] 王中和,涂文勇.口腔颌面-头颈肿瘤放射治疗学[M]. 上海：上海世界图书出版公司, 2013:209-210.

[2] 孔月虹,丁继平,涂文勇.肿瘤放疗对免疫作用影响[J]. 国际肿瘤学杂志, 2015, 42(6):448-451.

[3] Burnette B, Weichselbaum RR. Radiation as an immune modulator[J]. Semin Radiat Oncol, 2013, 23(4):273-280.

[4] Gaipl US, Multhoff G, Scheithauer H, et al. Kill and spread the word: stimulation of antitumor immune responses in the context of radiotherapy[J]. Immunotherapy, 2014, 6(5):597-610.

[5] 黄淑晶,王希成.放射治疗对肿瘤患者免疫功能的影响[J].临床医学工程, 2012, 19(1):151-154.

[6] Goodridge HS, Reyes CN, Becker CA, et al. Activation of the innate immune receptor Dectin-1 upon formation of a'phagocytic synapse'[J]. Nature, 2011, 472(7344): 471-475.

[7] Gao C, Li R, Wang S. Ulinastatin protects pulmonary tissues from lipopolysaccharide-induced injury as an immunomodulator[J]. J Trauma Acute Care Surg, 2012, 72(1):169-176.

[8] Apetoh L, Ghiringhelli F, Tesniere A, et al. Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy[J]. Nat Med,2007,13(9):1050-1059.

[9] Yu Q, Nie SP, Wang JQ, et al. Toll-like receptor 4 mediates the antitumor host response induced by Ganoderma atrum polysaccharide[J]. J Agric Food Chem, 2015, 63(2):517-525.

[10] Walker JA, Oliphant CJ, Englezakis A， et al. Bcl11b is essential for group 2 innate lymphoid cell development[J]. J Exp Med, 2015, 212(6):875-882.

[11] Gil J, Busto EM, Garcillán B, et al. A leaky mutation in CD3D differentially affects αβ and γδ T cells and leads to a Tαβ-Tγδ+B+NK+human SCID[J]. J Clin Invest, 2011, 121(10):3872-3876.

[12] Rossy J, Owen DM, Williamson DJ, et al. Conformational states of the kinase Lck regulate clustering in early T cell signaling[J]. Nat Immunol, 2013,14(1):82-89.

[13] Rajasekaran K, Kumar P, Schuldt KM, et al. Signaling by Fyn-ADAP via the Carma1-Bcl-10 -MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells[J]. Nat Immunol, 2013,14(11):1127-1136.

[14] McLane LM, Banerjee PP, Cosma GL, et al. Differential localization of T-bet and Eomes in CD8T cell memory populations[J]. J Immunol, 2013,190(7):3207-3215.

[15] Eric A, Juranic Z, Tisma N, et al. Radiotherapy-induced changes of peripheral blood lymphocyte subpopulations in cervical cancer patients: relationship to clinical response[J]. J BUON, 2009, 14(1):79-83.

选择文件类型/文献管理软件名称

选择包含的内容

放疗早期对口腔颌面部肿瘤患者免疫功能影响的临床研究

Effects of Radiotherapy on the Immune Function in Patients with Oral and Maxillofacial Cancer

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张雨菡，韩雯斐，孟博，苏俭生，肖凡. 调控小鼠腮腺分泌中枢神经系统相关核团的分布[J]. 《口腔颌面外科杂志》, 2019, 29(3): 121-.
[2]	马旭辉，王丽珍，任国欣，吴云腾，顾挺，郭伟. SOX10作为原发性口腔黏膜恶性黑色素瘤分子诊断指标的初步研究[J]. 《口腔颌面外科杂志》, 2019, 29(3): 131-.
[3]	范德生，甄蕾，汪黎明. miRNA-31对糖尿病小鼠来源骨髓间充质干细胞体外成骨分化的影响[J]. 《口腔颌面外科杂志》, 2019, 29(3): 137-.
[4]	郭泽鸿，周磊，徐淑兰，丁祥龙，赵春萍, 卢灿. 改良式骨劈开术水平骨增量对软硬组织的影响[J]. 《口腔颌面外科杂志》, 2019, 29(3): 142-.
[5]	张庆福，牛璐，符国才，聂恒金，吕春堂. 中段桥接式钛合金全下颌骨植入体的设计制作研究[J]. 《口腔颌面外科杂志》, 2019, 29(3): 148-.
[6]	吴东，林兆楠，林彦君，周麟，游冬冬，黄文秀，陈永辉. 自体牙来源骨移植材料在口腔种植中应用的短期临床观察[J]. 《口腔颌面外科杂志》, 2019, 29(3): 152-.
[7]	冯海楠，王淼，常晓峰，杜良智，周慧霞，李晓红，刘少丽，贺龙龙. 上颌窦底提升术后种植修复的十年临床回顾分析[J]. 《口腔颌面外科杂志》, 2019, 29(3): 159-.
[8]	施雄，李生娇，周剑萍，张利，李百龙. 锥形束CT在牙内陷诊断中的应用[J]. 《口腔颌面外科杂志》, 2019, 29(3): 164-.
[9]	岳广娜，王佐林. 石墨烯家族生物相容性的研究进展[J]. 《口腔颌面外科杂志》, 2019, 29(3): 169-.
[10]	孙睿男，王佐林. 单细胞转录组测序技术进展及其在间充质干细胞研究中的应用#br#[J]. 《口腔颌面外科杂志》, 2019, 29(3): 174-.
[11]	李宏权1，宋扬1，李志耀1，吴玉波1，钱文昊1，周曾同2. 丹酚酸B及其磷脂复合纳米粒抑制口腔鳞癌和白斑细胞株的作用[J]. 《口腔颌面外科杂志》, 2018, 28(6): 314-321.
[12]	冯大军，蒋勇，孙云峰. 改良下唇切口进路切除口腔恶性肿瘤[J]. 《口腔颌面外科杂志》, 2018, 28(4): 215-218.
[13]	王佐林，王方. 创新发展，求同存异 ——“争鸣与共识”种植论坛（第四季）学术共识[J]. 《口腔颌面外科杂志》, 2018, 28(2): 61-.
[14]	万会璇，蔡明详，孙瑶. 局部激活破骨细胞对大鼠应用双膦酸盐后拔牙创愈合的影响[J]. 《口腔颌面外科杂志》, 2018, 28(2): 65-.
[15]	李威，杨旭东，王志勇，卢晓林，王洋，王育新. 数字化技术辅助颌骨截骨及颌面修复的临床应用[J]. 《口腔颌面外科杂志》, 2018, 28(2): 75-.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

放疗早期对口腔颌面部肿瘤患者免疫功能影响的临床研究

Effects of Radiotherapy on the Immune Function in Patients with Oral and Maxillofacial Cancer

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价