负载不同载药胶束纳米纤维膜的构建及其体外抗炎作用比较研究

doi:10.3969/j.issn.1005-4979.2018.03.002

摘要/Abstract

摘要： 目的：研究分别负载免疫抑制剂（SP600125、SB203580和PD98059）聚合物胶束的纳米纤维膜，在体外对LPS（脂多糖）诱导的牙周膜细胞（periodontal ligament cells，PDLCs）的抗炎作用的影响，并进行比较。方法：通过薄膜水化法制备载药胶束（SP-PMs、SB-PMs、PD-PMs），并通过静电纺丝技术构建纳米纤维膜，使得免疫抑制剂负载其中。通过细胞学实验检测纳米纤维膜的生物相容性，和其对炎症相关基因及蛋白的表达影响。结果：扫描电镜显示纳米纤维呈线型结构，且纤维直径分布集中，激光共聚焦显示载药胶束负载于纤维中；细胞的增殖不受纳米纤维膜的影响；体外实验表明3种不同的载药纳米纤维膜均能抑制炎症因子的表达，其中负载SP-PMs、SB-PMs的纤维抗炎效果明显好于PD-PMs组。结论：负载SP-PMs或SB-PMs的纳米纤维膜显示出良好的抗炎效果，这为牙周炎的治疗提供了新的展望方向。

关键词: 载胶束纳米纤维膜, 免疫抑制剂, 牙周炎, 牙周膜细胞, 抗炎作用

Abstract: Objective: The study aimed to assess and make a comparison between nanofiber mats encapsulated with three drug-loaded (SP600125, SB203580 and PD98059) polymeric micelles respectively on the anti-inflammatory effect in vitro. Methods: The drug-loaded micelles (SP-PMs, SB-PMs and PD-PMS) were prepared by the thin-film hydration method, and then encapsulated into nanofibers by using electrospinning technique. Cytotoxicity and the expression levels of inflammation-related genes MMP-2, MMP-9 as well as corresponding proteins were performed by periodontal ligament cells ( PDLCs). Results: The TEM images revealed the obtained nanofibers was line shape, and showed a narrow distribution range. The CLSM images verified that the drug-loaded micelles were encapsulated into nanofibers uniformly. The co-culturing of nanofiber mats and PDLCs demonstrated a good biocompatibility. In vitro anti-inflammation test illustrated that three drug-loaded nanofiber mats could both significantly decline the expression of MMP-2 and MMP-9 at gene and protein levels, and the inhibitory effects of SP-PMs and SB-PMs were better than PD-PMs group. Conclusion: The results suggested that SP-PMs or SB-PMs encapsulated nanofiber mat showed great anti-inflammatory effect in vitro. The present article presents an alternative technique that may be considered by periodontologists.

Key words: polymeric micelles loaded nanofiber mats, immunosuppressor, periodontitis, periodontal ligament cells, anti-inflammatory effect

中图分类号:

R782.4

刘肖晨，苏俭生. 负载不同载药胶束纳米纤维膜的构建及其体外抗炎作用比较研究[J]. 《口腔颌面外科杂志》, doi: 10.3969/j.issn.1005-4979.2018.03.002.

LIU Xiao-Chen, SU Jian-Sheng. The Fabrication of Polymeric Micelles Loaded Core-Shell Structured Nanofiber Mats and Their Effects on Anti-Inflammation in Vitro[J]. 《Journal of Oral and Maxillofacial Surgery》, doi: 10.3969/j.issn.1005-4979.2018.03.002.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2018/V28/I3/127

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