摘要：

目的：探究茶黄素-3, 3′-双没食子酸酯（theaflavin-3, 3′-digallate，TF3）对骨质疏松的作用及其机制。方法：建立小鼠去卵巢（ovariectomy，OVX）骨质疏松模型，注射剂量为1 mg/kg和10 mg/kg的TF3，3个月后取股骨进行微型CT（Micro-CT）分析。体外应用过氧化氢（H₂O₂）建立MC3T3-E1细胞氧化应激模型，并分别加入0、1、10 μmol/L 的TF3，检测TF3对细胞活性、细胞内活性氧（reactive oxygen species，ROS）含量和抗氧化基因表达的影响。结果：Micro-CT分析结果表明，TF3能降低骨质疏松的骨小梁丢失程度，且随TF3的剂量增加，效果越明显。进一步的体外实验结果表明，加入H₂O₂后，MC3T3-E1的细胞活性明显降低，ROS含量明显增加；而同时加入TF3后， ROS水平明显下降，抗氧化基因Nrf2及其下游的HO-1、CAT表达水平增加，且随TF3浓度增加，效果越明显。结论：TF3可以降低OVX引起的骨丢失程度，其作用可能与TF3具有细胞氧化损伤的保护作用有关。

关键词: 茶黄素-3, 3′-双没食子酸酯, MC3T3-E1细胞, 氧化应激, 骨质疏松

Abstract:

Objective: To investigate the effect of theaflavin-3, 3′-digallate(TF3) on osteoporosis and its mechanism. Methods: An osteoporosis model in mice was established by ovariectomy(OVX), and TF3 was injected at a gradient concentration (1 mg/kg, 10 mg/kg). After 3 months, mice femurs were taken for Micro-CT analysis. H₂O₂ was used to establish MC3T3-E1 cells oxidative stress model in vitro. 0, 1, and 10 μmol/L TF3 were added to detect the effects of TF3 on cell activity, the level of intracellular reactive oxygen species (ROS), and the expression of antioxidant genes. Results: Micro-CT analysis showed that TF3 could reduce the volume of trabecular bone loss in osteoporosis, and the degree of bone loss manifested a positive correlation with the increase of the dose of TF3. Further in vitro experiments showed that the activity of MC3T3-E1 cells significantly decreased and the ROS content significantly increased after the addition of H₂O₂. The addition of TF3 significantly reduced the ROS level and increased the expression level of antioxidant gene Nrf2 and its downstream HO-1 and CAT, and the effect became more obvious with the increase of TF3 concentration. Conclusion: TF3 can reduce the degree of bone loss caused by OVX, which may be related to the protective effect of TF3 on cellular oxidative damage.

Key words: theaflavin-3, 3′-digallate (TF3), MC3T3-E1 cells, oxidative stress, osteoporosis

中图分类号: 

• R782