环杷明对人腮腺多形性腺瘤细胞凋亡的影响

doi:10.3969/j.issn.1005-4979.2012.03.003

《口腔颌面外科杂志》 ›› 2012, Vol. 22 ›› Issue (3): 163-167. doi: 10.3969/j.issn.1005-4979.2012.03.003

环杷明对人腮腺多形性腺瘤细胞凋亡的影响

欧学平¹，肖翠翠¹，宋萌¹，肖忠明²，韦芳²，洪艳²

1.上海交通大学附属上海第一人民医院口腔科，2.实验中心，上海　200080

出版日期:2012-06-28 上线日期:2012-08-30
通讯作者: 宋萌，教授. E-mail:smgjzh160@sohu.com
作者简介:欧学平（1981—），男，湖北黄冈市人，硕士研究生. E-mail:omigaso@126.com
基金资助:
上海市科学技术委员会资助项目（08JC1412900）；上海市重点学科建设项目（S30206-KF05）

Cyclopamine Induces Apoptosis in Human Salivary Pleomorphic Adenoma Cells

OU Xue-ping¹, XIAO Cui-cui¹, SONG Meng¹, XIAO Zhong-ming², WEI Fang², HONG Yan²

1.Department of Stomatology, 2.Experimental Research Center, Shanghai Municipal First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China

Published:2012-06-28 Online:2012-08-30

摘要/Abstract

摘要： 目的：探讨环杷明（cyclopamine）诱导人腮腺多形性腺瘤细胞凋亡的作用及其对Gli-2和Bcl-2表达的影响。方法：用10 μmol∕L环杷明处理人腮腺多形性腺瘤细胞，48 h后倒置显微镜下观察细胞数量及形态变化；48 h后实时定量PCR检测实验组（环杷明组）和空白对照组、DMSO对照组的Gli-2、Bcl-2 mRNA表达水平差异；24 h后流式细胞术观察细胞凋亡。结果：人腮腺多形性腺瘤细胞用10 μmol∕L 环杷明作用48 h后倒置显微镜下可见细胞数量减少、细胞核固缩或碎裂、核仁变形等典型的细胞凋亡形态学变化；环杷明组与空白对照组、DMSO对照组相比，可以显著下调Gli-2、Bcl-2 mRNA表达（P<0.01）；环杷明组的细胞凋亡率明显高于空白对照组和DMSO对照组（P<0.01）。结论：环杷明可诱导人腮腺多形性腺瘤细胞凋亡，其作用机制可能与下调Gli-2、Bcl-2 mRNA表达，活化细胞凋亡的线粒体途径有关。

关键词: 腮腺多形性腺瘤细胞, 细胞凋亡, 环杷明

Abstract: Objective: The purpose of this study was to examine whether cyclopamine is related to apoptosis and growth inhibition of human salivary pleomorphic adenoma (HSPA) cells. Methods: HSPA cells were treated with cyclopamine.Observed the cells quantity change and morphology after 48 h. Cells cultured with DMSO 0.08% or void were used as control. Gli-2 and Bcl-2 mRNA expression was examined by Real-time PCR after 48 h. Flow cytometry was used to analyze cell apoptosis after 24 h. Experiment results are presented as x±s. Statistical analysis was undertaken with SAS 9.13. The differences among the groups were identified by one-way ANOVA. Significance was accepted at P<0.01. Result: The number of cyclopamine treated HSPA cells was significantly decreased. Cyclopamine treated (10 μmol/L) HSPA cells showed nuclear psychosis or fragmentation, chromatospherite disfiguration with apoptoticmorphology. The expression levels of Gli-2 and Bcl-2 mRNA in 10 μmol∕L cyclopamine treated group was down-regulated significantly compare with blank and DMSO control groups (P<0.01). Apoptosis rate of 10 μmol∕L cyclopamine treatment group was significantly higher than blank and DMSO control group (P<0.01). Conclusion: Cyclopamine appears to induce growth inhibition and apoptosis in human salivary pleomorphic adenoma cells. The mechanism may be related to down-regulation of Gli-2 and Bcl-2 mRNA, which activates the mitochondrial apoptotic pathways.

Key words: pleomorphic adenoma cell, apoptosis, cyclopamine

中图分类号:

R739.87

欧学平，肖翠翠，宋萌，肖忠明，韦芳，洪艳. 环杷明对人腮腺多形性腺瘤细胞凋亡的影响[J]. 《口腔颌面外科杂志》, 2012, 22(3): 163-167.

OU Xue-ping, XIAO Cui-cui, SONG Meng, XIAO Zhong-ming, WEI Fang, HONG Yan. Cyclopamine Induces Apoptosis in Human Salivary Pleomorphic Adenoma Cells[J]. 《Journal of Oral and Maxillofacial Surgery》, 2012, 22(3): 163-167.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2012/V22/I3/163

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