Abstract: Objective: This study was designed to observe the effects of liquid nitrogen cryotherapy combined with Nd:YAG laser radiation on veins in rabbit ears, so as to assess its feasibility in the treatment of venous malformations. Methods: Sixty-five rabbits were randomly divided into 4 groups：Group A, Group B and Group C were experimental groups (n=20), Group D was a blank control group (n=5). The central postauricolar veins of rabbits were taken as the models. Group A, veins were frozen with liquid nitrogen and immediately irradiated by Nd:YAG laser; Group B, veins were frozen by liquid nitrogen only; Group C, veins were irradiated by Nd:YAG laser only. Group D, veins retained in blank control. After 1, 3, 7, 14 and 21 days of disposal, the veins were examined under gross observation, light microscopy, and transmission electron microscopy (SEM). Results: In Group A, damages to endothelial cells, vascular smooth muscles and the architecture of structures of veins were obvious, thrombosis appeared and finally resulted in venous obstruction. Group B and Group C, vascular endothelial cell injuries and thrombosis could be found, but thrombosis was gradually dissolved and veins were repaired. Conclusion: Liquid nitrogen cryotherapy combined with Nd:YAG laser radiation could result in major injury to the auricular veins of rabbits and may be applied for venous malformation treatment.

Key words:  , liquid nitrogen/ cryotherapy/  , Nd:YAG laser/  , the veins of rabbit ears

摘要：
目的: 观察液氮冷冻联合Nd:YAG激光照射对兔耳静脉的作用，评估该方法治疗静脉畸形的可行性。方法：将65只白兔随机分为4组。A、B、C为实验组，每组20只；D组为空白对照组，5只。以兔耳背中央静脉为实验模型，A组行液氮冷冻及Nd:YAG激光照射，B组行液氮冷冻，C组行Nd:YAG激光照射，D组不做处理。分别于实验处置后1、3、7、14、21 d对兔耳背中央静脉进行大体、光镜及电镜观察。结果：A组静脉血管内皮细胞、管壁平滑肌及构架结构均有明显损伤破坏，管腔内血栓形成，最终静脉闭锁。B、C组虽有血管内皮细胞损伤及血栓形成，但随后血栓逐渐溶解，血管修复。结论：冷冻联合激光照射对兔耳静脉有较强的损伤作用，具有治疗静脉畸形的可能性。

关键词:  , 液氮/ 冷冻/ Nd:YAG激光/ 兔耳静脉

CLC Number: 

• R732.2