Abstract: Objective: The aim of this study was to evaluate the capacity of temperature and  storage media in maintaining the viability of human periodontal ligament fibroblasts（PDLF）. Methods: PDLF in vitro were kept in whole milk, Hank's balanced salt solution (HBSS),  1 mmol/L alendronate in HBSS （HBSS-1）,  5 mmol/L glutathione in HBSS （HBSS-2）, 10 ng/mL basic fibroblast growth factor in HBSS（HBSS-3）respectively. The temperature of solutions were at 4℃ and 20℃ respectively. After 3, 6, 24, 48 and 72 h in storage media, cell viability of PDLF were examined by CCK-8 assay. Results: At 4℃, the PDLF viabilities in every storage solution showed a downward trend with time, The milk exhibited  better than other storage media in maintaining cell viability at different storage time, especially at 6 h(P<0.05). At 20℃, the PDLF viabilities in every storage media were still decreasing over time. Among them, HBSS-3 had a relatively slow decline. HBSS and HBSS-3 were better than others  in maintaining cell viability at different storage time. HBSS-3 was significantly better than HBSS-1 at 72 h(P<0.05). In general, the performance of HBSS-1, HBSS-2 and HBSS were not influenced by storage temperature. However, the higher temperature decreased the effectiveness of whole milk, especially at 6 and 24 h(P<0.05). But, favored the HBSS-3, especially at 48 and 72 h (P<0.05). Conclusion: Whole milk may be an effective and convenient storage medium for avulsed teeth in a short time period and at lower temperature condition. HBSS and HBSS-3 may be suitable for long term storage in normal temperature.

Key words:  , avulsion, periodontal ligament fibroblast viability, alendronate, reduced glutathione, basic fibroblast growth factor

摘要： 目的：比较5种离体牙储存液，在不同温度、不同储存时间条件下，体外维持人牙周膜成纤维细胞（periodontal ligament fibroblasts, PDLF）活性的能力，为外伤后离体牙的保存提供实验依据。方法：选取5种离体牙存储液：牛奶、汉克斯液（Hank's balanced salt solution，HBSS）、HBSS1（HBSS+阿仑膦酸钠）、HBSS2（HBSS+还原型谷胱甘肽）、HBSS3（HBSS+碱性成纤维细胞生长因子），用cck-8试剂测定人PDLF不同温度下，在上述溶液中保存不同时间后的细胞活性。结果：4℃条件下，各实验组的PDLF细胞活性随时间延长均呈下降趋势。在不同时间点，牛奶组均表现出相对较高的细胞活性，尤其在6 h时与其他实验组相比，差异有统计学意义（P<0.05）。20℃条件下，各实验组的PDLF细胞活性随时间延长也呈下降趋势,其中HBSS3组活性下降相对缓慢。在不同时间点，HBSS组和HBSS3组均表现出相对较高的细胞活性，尤其在72 h时，HBSS3组与HBSS1组相比，差异有统计学意义（P<0.05）。总体上，HBSS组、HBSS1组和HBSS2组的存储效果不受温度影响，而牛奶组和HBSS3组的存储效果与温度有关。HBSS3组PDLF细胞活性20℃时高于4℃，其中48 h、72 h差异有统计学意义（P<0.05），而牛奶组PDLF细胞活性20℃时低于4℃，其中6 h、24 h差异有统计学意义（P<0.05）。结论：牛奶可作为一种方便有效的储存介质用于脱位牙的短期保存，且低温时更有优势。当脱位牙需要长期储存时，HBSS和HBSS3可作为合适的存储液。并且，HBSS在4℃和20℃时存储效果相当，而HBSS3在室温下优势更明显。

关键词:  牙脱位；  , 牙周膜成纤维细胞；  , 阿仑膦酸钠；  , 谷胱甘肽；  , 碱性成纤维细胞生长因子

CLC Number: 

• R782.4