Abstract:  Objective: The present study was proposed to investigate in vivo osteogenic potential of bone marrow stromal cells(BMSCs) in negative pressure treated β-Tricalcium phosphate(β-TCP) scaffolds. Methods: TCP was dealt with the syringe system (made for a subatmospheric pressure), cocultured or not cocultured with BMSCs, and differentiate into osteogenic direction for 2 weeks. BMSCs sheet was packed into TCP scaffolds and  embedded subcutaneously in nude mice for 8 weeks. Animals were sacrificed, osteogenic potential were observed histologically by HE, and Masson stain. Results:  TCP cocultured with BMSCs packed with cell sheets showed better osteogenic potential in vivo . Conclusion: Because of the formation of extracellular matrix, the negative pressure and cell sheets may promote cell colonization and improve bone formation in vivo.

Key words: tissue engineering, bone regeneration, bone marrow stromal cells, cell sheet, negative pressure system

摘要： 目的： 研究经过负压处理的TCP支架材料与细胞复合后的体内成骨能力。方法：将TCP支架材料负压处理，和骨髓基质细胞（BMSCs）细胞共培养一段时间后，成骨诱导2周，电镜下观察支架材料和细胞复合情况。再将该复合物与BMSCS细胞膜片复合，植入到裸鼠皮下，8周后作组织切片，HE染色和Masson染色，观察其体内成骨能力。结果：负压（有细胞）+膜片组异位移植8周后，内部可见大量胶原纤维和成骨纤维，在团块样组织内部可见大量成骨细胞，并有血管形成。对照组仅有个别细胞密集区，胶原纤维和成骨纤维较少。空白组未见类似情况。结论：经过负压+骨髓基质细胞膜片处理的TCP支架材料，在体内有成骨潜能。

关键词: 组织工程, 骨再生, 骨髓基质细胞, 细胞膜片, 负压

CLC Number: 

• R782.2