The Osseointegration and Degradation Behavior of Pure Magnesium PLGA Film in Vivo

doi:10.3969/j.issn.1005-4979.2020.05.003

《Journal of Oral and Maxillofacial Surgery》 ›› 2020, Vol. 30 ›› Issue (5): 279-284. doi: 10.3969/j.issn.1005-4979.2020.05.003

• Basic Scientific Study • Previous Articles Next Articles

The Osseointegration and Degradation Behavior of Pure Magnesium PLGA Film in Vivo

ZHENG Kaining¹(), XIAO Yue¹, LI Muqin²(), ZHANG Erlin⁴(), YAO Wei³, CHU Minghui¹

¹ Department of Prosthodontics, Affiliated Stomatological Hospital, Jiamusi University, Jiamusi 154007, Heilongjiang Province
² Key Research Laboratory of Biomedical Materials in Heilongjiang Province, Jiamusi University, Jiamusi 154007, Heilongjiang Province
³ Life Science Experiment Center of Jiamusi University, Jiamusi 154007, Heilongjiang Province
⁴ Key Laboratory of Material Anisotropy and Texture, Ministry of Education, School of Materials Science and Engineering, Northeastern University, Liaoning 110004, Shenyang Province, China

Received:2019-12-28 Revised:2020-03-26 Published:2020-10-28 Online:2020-10-27

纯镁载聚乳酸-乙醇酸膜层的体内骨结合与降解行为

郑凯宁¹(), 肖月¹, 李慕勤²(), 张二林⁴(), 姚潍³, 初明慧¹

¹ 黑龙江佳木斯大学附属口腔医院修复科，黑龙江佳木斯 154007
² 佳木斯大学黑龙江省高校生物医学材料重点研究实验室，黑龙江佳木斯 154007
³ 佳木斯大学生命科学实验中心，黑龙江佳木斯 154007
⁴ 东北大学材料科学与工程学院材料各向异性与织构教育部重点实验室，辽宁沈阳 110004

通讯作者: 李慕勤，教授. E-mail: jmsdxlimuqin@163.com；张二林，教授. E-mail: zhangel@atm.neu.edu
作者简介:
郑凯宁（1994—），男，黑龙江五常人，医师，硕士. E-mail:1439907278@qq.com
基金资助:
国家自然科学基金(31370979)

Abstract

Abstract:

Objective: To investigate the effect of pure magnesium loaded with poly (lactic-co-glycolic acid）（PLGA) on osseointegration capacity and degradation rate in vivo. Methods: Pure magnesium loaded with micro arc oxidation (MAO) coating was used as control group(MAO group) and pure magnesium loaded with PLGA coating was used as experimental group (PLGA group). They were implanted into the mandible of rabbits. After 2, 4, 8 and 12 weeks, the rabbits were killed, and the mandible was cut off without decalcification. Series examinations of scanning electron microscope(SEM), CBCT, Micro-CT, and laser confocal microscope were performed. Results: CBCT observation showed that PLGA group had smaller low-density images and better osseointegration than MAO group. Micro-CT results showed that the degradation rate of PLGA group was lower than that of MAO group. SEM and laser confocal microscope results showed that the ability of promoting new bone formation in PLGA group was stronger than that in MAO group. Conclusion: Pure magnesium loaded with PLGA film can promote osseointegration and reduce the degradation rate of magnesium matrix.

Key words: pure magnesium, micro arc oxidation, poly (lactic-co-glycolic acid), osseointegration, degradation rate

摘要：

目的： 探讨纯镁载聚乳酸-乙醇酸共聚物[poly（lactic-co-glycolic acid），PLGA]对体内骨结合能力及降解速率的影响。方法： 纯镁微弧氧化（micro arc oxidation，MAO）膜层为对照组（MAO组），纯镁载PLGA膜层为实验组（PLGA组），将2种涂层的镁钉植入家兔下颌骨内。分别于2、4、8和12周后分期处死家兔，在不脱钙的情况下截取载钉的下颌骨，并进行锥形束CT（CBCT）、微型CT（Micro-CT）、激光共聚焦显微镜和扫描电镜检测。结果： CBCT 结果显示，PLGA组较MAO组低密度影像小，骨结合好；Micro-CT结果显示，PLGA组较MAO组降解速率低；扫描电镜和激光共聚焦显微镜可见PLGA组促新骨生成能力强于MAO组。结论： 纯镁载PLGA膜层能够促进骨结合，并降低镁基体的降解速率。

关键词: 纯镁, 微弧氧化, 聚乳酸-乙醇酸共聚物, 骨结合, 降解速率

CLC Number:

R782

ZHENG Kaining, XIAO Yue, LI Muqin, ZHANG Erlin, YAO Wei, CHU Minghui. The Osseointegration and Degradation Behavior of Pure Magnesium PLGA Film in Vivo[J]. 《Journal of Oral and Maxillofacial Surgery》, 2020, 30(5): 279-284.

郑凯宁, 肖月, 李慕勤, 张二林, 姚潍, 初明慧. 纯镁载聚乳酸-乙醇酸膜层的体内骨结合与降解行为[J]. 《口腔颌面外科杂志》, 2020, 30(5): 279-284.

/ / Recommend / Download Citations

URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2020.05.003

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2020/V30/I5/279

Figures/Tables 6

Figure 1 CBCT images showing magnesium implants in MAO and PLGA groups of rabbits at 2,4,8 and 12 weeks

Figure 2 Micro-CT images of magnesium implants and magnesium plates at 4 and 8 weeks

Table 1 The magnesium implants trabecular bone data of two groups at 4 and 8 weeks

组别	4周			8周
组别	骨小梁厚度/mm	骨小梁数量/(1/mm)	骨小梁间距/mm	骨小梁厚度/mm	骨小梁数量/(1/mm)	骨小梁间距/mm
MAO组	0.08	0.92	1.01	0.09	0.67	1.40
PLGA组	0.11	1.64	0.50	0.11	1.50	0.56

Figure 3 Residual volume of magnesium matrix before implantation and 4, 8 and 12 weeks after implantation

Figure 4 Confocal laser images of magnesium implants in MAO and PLGA groups at 2, 4 and 8 weeks

Figure 5 Morphology characterization and element analysis by SEM of magnesium implants in MAO and PLGA groups at 4 and 8 weeks

References 15

[1]	周伟, 孙庚林, 周健, 等. 下颌角骨折坚强内固定后应力遮挡效应的三维有限元分析[J]. 实用口腔医学杂志, 2011, 27(3): 320-324.
[2]	胡文雄, 郭祥. 钛合金板置入内固定修复老年肱骨远端骨折的生物相容性[J]. 中国组织工程研究, 2016, 20(13): 1888-1894.
[3]	Chen JX, Tan LL, Yu XM, et al. Mechanical properties of magnesium alloys for medical application: a review[J]. J Mech Behav Biomed Mater, 2018, 87: 68-79. doi: S1751-6161(18)31032-4 pmid: 30041141
[4]	Staiger MP, Pietak AM, Huadmai J, et al. Magnesium and its alloys as orthopedic biomaterials: a review[J]. Biomaterials, 2006, 27(9): 1728-1734. doi: 10.1016/j.biomaterials.2005.10.003 pmid: 16246414
[5]	明堃, 李德超, 李慕勤, 等. 纯镁复合硅烷/植酸载锌膜层的细胞相容性实验研究[J]. 口腔颌面外科杂志, 2018, 28(1): 14-20. doi: 10.3969/j.issn.1005-4979.2018.01.003
[6]	宁闯明, 崔学军, 王淋, 等. 氧化时间对AZ31B镁合金微弧氧化涂层结构及性能的影响[J]. 表面技术, 2019, 48(7): 158-165.
[7]	李慕勤, 刘惠萍, 刘苗, 等. 褪黑素对纯钛超声微弧氧化膜层体内植入骨结合的影响[J]. 口腔颌面外科杂志, 2016, 26(5): 346-352.
[8]	Kapoor DN, Bhatia A, Kaur R, et al. PLGA: a unique polymer for drug delivery[J]. Ther Deliv, 2015, 6(1): 41-58. doi: 10.4155/tde.14.91 pmid: 25565440
[9]	Astete CE, Sabliov CM. Synthesis and characterization of PLGA nanoparticles[J]. J Biomater Sci Polym Ed, 2006, 17(3): 247-289. doi: 10.1163/156856206775997322 URL
[10]	段平国, 郭润生, 余兴元, 等. 单一生物材料聚乳酸/羟基乙酸共聚物构建组织工程骨软骨的可行性[J]. 中国组织工程研究, 2017, 21(34): 5423-5429.
[11]	Li RW, Kirkland NT, Truong J, et al. The influence of biodegradable magnesium alloys on the osteogenic differentiation of human mesenchymal stem cells[J]. J Biomed Mater Res A, 2014, 102(12): 4346-4357. doi: 10.1002/jbm.a.35111 pmid: 24677428
[12]	Zhao DW, Witte F, Lu FQ, et al. Current status on clinical applications of magnesium-based orthopaedic implants: a review from clinical translational perspective[J]. Biomaterials, 2017, 112: 287-302. doi: S0142-9612(16)30558-0 pmid: 27770632
[13]	黄晶晶, 任伊宾, 张炳春, 等. 可降解镁植入材料表面涂层的制备及其性能[J]. 中国有色金属学报, 2007, 17(9): 1465-1469.
[14]	陈伟, 张瑞鹏, 邵佳申, 等. 新型生物可降解PLGA-Mg材料的制备与初步研究[J]. 河北医科大学学报, 2016, 37(12): 1466-1469.
[15]	Tian P, Liu XY. Surface modification of biodegradable magnesium and its alloys for biomedical applications[J]. Regen Biomater, 2015, 2(2): 135-151. doi: 10.1093/rb/rbu013 pmid: 26816637

Please choose a citation manager

Content to export