摘要： 目的： 探讨在SD大鼠下颌骨临界性骨缺损(critical size bone defect,CSD)与自愈性骨缺损修复过程中，各时相点干细胞因子(stem cell factor, SCF)的表达情况。方法： ①分别在36只SD大鼠双侧下颌骨的下颌角部制造临界性骨缺损(直径5 mm)和自愈性骨缺损(直径2 mm)模型，实验动物分为6组，在术后第1、3、5、7、14、21天分别处死；②利用免疫组织化学方法检测骨缺损区各时相点SCF的表达情况；③利用Western印迹法检测骨缺损区各时相点SCF存在与否，并进行半定量分析。结果： 成功构建了大鼠双侧下颌骨的临界性骨缺损和自愈性骨缺损模型。免疫组织化学结果显示，骨缺损愈合的各时相点SCF均有较高的表达，但SCF在临界性骨缺损组各时相点的表达，均低于自愈性骨缺损组。结论： SCF在骨缺损修复中发挥重要的作用。

关键词:  , 干细胞生长因子, 临界性骨缺损, 自愈性骨缺损, 鼠

Abstract: Objective: The current paper was aimed to detect the expression of stem cell factor (SCF) during the repair process of rats′ mandibular defects.  Methods: 36 SD rats were used in this study. In one side of the mandible , critical size bone defect (CSD) in 5 mm dimension was established. A self-healing bone defect in 2 mm dimension was created in the contralateral side of the mandible. Animals were randomly divided into 6 groups and sacrificed on 1, 3, 5, 7, 14, 21 d after surgery respectively. Expression of SCF during the regeneration process were determined by immunohistochemistry and Western blot. Data were expressed as x+s by SPSS 13.0 software. We used Student′s t test to determine the significance of difference between groups.  Significance was set at P<0.05. Results: The expression of SCF genes was detected in both groups at all times, and expression of SCF in CSD group was weaker  than those in self-healing bone defect group at the same time(P<0.05). The results of western blot also confirmed the results by immunohistochemistry. Conclusion: SCF plays an important role in bone defect repair.

Key words: stem cell factor, critical size defect, self-healing size defect, rat

中图分类号: 

• R782.4