摘要： 目的：通过观察整合素连接激酶（Integrin-linked kinase, ILK）与一氧化氮合酶（nitric oxide synthase，NOS）在涎腺腺样囊性癌（salivary adenoid cystic carcinoma, SACC）中的定位及表达，探讨ILK与NOS在SACC中的作用。方法：对2006-2012年吉林医药学院附属医院病理科30例SACC存档蜡块，和25例非肿瘤疾病的正常涎腺组织（对照组）行免疫组化分析，观察ILK与NOS在SACC中的表达分布情况。利用SPSS13.0统计软件分析ILK与NOS的差异性及相关性。结果：在SACC中，ILK阳性率为76.67%，eNOS阳性率为63.33%，iNOS阳性率为80%。ILK实验组与对照组比较，P<0.05；eNOS实验组与对照组比较，P>0.05；iNOS实验组与对照组比较，P<0.05。ILK与eNOS在SACC中的表达不存在相关性；ILK与iNOS在SACC中的表达存在正相关，r=0.512。结论：ILK与iNOS在SACC中的表达存在正性相关，可能通过iNOS信号转导途径启动VEGF的表达，促进肿瘤早期的血管发生和形成。

关键词: 整合素连接激酶（ILK）, 一氧化氮合酶（NOS）, 涎腺腺样囊性癌（SACC）, 免疫组织化学, 相关性分析

Abstract: Purpose: To examine the expressions of integrin-linked kinase(ILK) and nitric oxide synthase (NOS) and its significance in SACC. Methods：30 cases of SACC from 2006 to 2012 were used to determine the expression of ILK and NOS by immunohistochemistry examination. The correlation of ILK and NOS were statistically analyzed by SPSS software. Results: The positive rate of ILK expression was 76.67%; The positive rate of eNOS expression was 63.33%; The positive rate of iNOS expression was 80%. ILK and iNOS expression manifested significant differences between experiment and control groups; but expression of eNOS did not show statistical difference. No positive correlation of expression of ILK and eNOS in SACC was confirmed. Expression of ILK were negatively correlated with iNOS expression in SACC, r=0.512. Conclusion:  The iNOS plays a role that promoting the growth of SACC and tumor angiogenesis, and eNOS effects are not obvious. ILK and iNOS positive correlation may start signal transduction pathways and motivate VEGF expression, thus promote early tumor angiogenesis and formation.

Key words: ILK, NOS, SACC, immunohistochemistry, correlation analysis

中图分类号: 

• R739.87