摘要： 目的：观察降钙素基因相关肽（calcitonin gene-related peptide，CGRP）在犬骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）向成骨细胞分化过程中，及应用BMSCs构建组织工程骨提升犬上颌窦底后骨组织中的表达情况。方法：体外分离培养犬BMSCs，通过成骨诱导采用实时荧光定量PCR（real-time quantitative polymerase chain reaction，Q-PCR）和免疫荧光技术检测CGRP在不同时间点的表达情况。犬上颌窦底提升术后，分别在两侧上颌窦底植入BMSCs+Bio-Oss骨粉（实验组）和Bio-Oss骨粉（对照组）。12周后处死并获取动物标本，对窦底区骨组织行CGRP免疫组化染色。结果：体外实验示CGRP表达量随成骨诱导分化呈时间依赖性递增（P<0.05）；上颌窦底提升实验组CGRP表达量明显高于对照组（P<0.05），且CGRP主要分布在骨再生代谢活跃区域。结论：CGRP表达量与骨再生过程密切相关，可考虑作为骨代谢活跃度的标志。

关键词: 降钙素基因相关肽, 上颌窦底提升术, 成骨诱导, 骨髓间充质干细胞, 犬

Abstract: Objective: To observe the expressive levels of calcitonin gene-related peptide (CGRP)  in bone marrow mesenchymal stem cells(BMSCs) during osteoinduction, and in the remodeled bone of maxillary sinus floor augmentation. Methods: In vitro, the BMSCs were isolated from Beagle dogs and were classified into osteogenic-induced group and non-induced group. Expression of the CGRP was detected by Q-PCR and immunofluorescence in the different time-points. In vivo, both right and left maxillary sinus of three healthy adult dogs were divided into experimental group and control group randomly. BMSCs complexed with Bio-Oss granules were applied for canine maxillary sinus augmentation models, while Bio-Oss granules alone served as controls. Immunohistochemical analysis were performed after 12 weeks on these bone substitutes. Results: Q-PCR and immunofluorescence  examinations demonstrated the expression of CGRP in osteogenic-induced group was increased in a time-dependent manner. After maxillary sinus floor elevation, the expressive levels of CGRP in experimental group were superior than that of control group (P<0.05). CGRP was mainly distributed in the region where osteogenesis metabolism was active. Conclusion: The expression of CGRP was related to the progress of bone regeneration, and it can be regarded as an indicator of the activity of osteogenesis metabolism.

Key words: calcitonin gene-related peptide, maxillary sinus augmentation, osteogenic induction, bone marrow mesenchymal stem cells, dog

中图分类号: 

• R782.2