摘要： 目的：观察siRNA沉默口腔癌耐长春新碱细胞株KB/VCR细胞COX-2基因后，对KB/VCR细胞增殖及侵袭能力的影响。方法：将KB/VCR细胞分为COX-2 siRNA组、阴性对照组及空白对照组，采用RT-PCR检测各组细胞COX-2 mRNA的表达，蛋白印迹法检测COX-2蛋白的表达，MTT法检测各组细胞的生长抑制率，Transwell小室测定各组细胞侵袭能力的变化。结果：COX-2 siRNA组细胞的COX-2蛋白和mRNA的表达明显低于阴性对照组和空白对照组(P<0.01)。COX-2 siRNA组细胞的生长抑制率明显高于阴性对照组和空白对照组(P<0.01)。COX-2 siRNA组细胞的侵袭能力也明显降低。结论：COX-2 siRNA能特异性沉默KB/VCR细胞的COX-2基因，使KB/VCR细胞生长得到抑制，并减弱其侵袭能力。

关键词: KB/VCR细胞, 环氧化酶-2, 小干扰RNA, 增殖, 侵袭

Abstract: Objective： To investigate the effect of small interfering RNA-mediated (siRNA-mediated) COX-2 gene silencing to change the proliferation and invasion behavior of KB/VCR cell lines. Methods： The KB/VCR cells were divided into 3 groups ,which were COX-2 siRNA group, negative control group, blank control group. The transcription of COX-2 gene was examined with RT-PCR. The protein expression of COX-2 was determined by Western blotting. MTT assay was used to analyze the proliferation of KB/VCR cells. The cell invasion was evaluated using a transwell assay. Results： COX-2 mRNA and protein levels were significantly reduced in COX-2 siRNA group (P<0.01). The growth inhibition of KB/VCR cells in COX-2 siRNA group was significantly higher than other groups (P<0.01). Meanwhile, the invasiveness of KB/VCR cells in COX-2 siRNA group was decreased remarkably. Conclusion： siRNA-mediated COX-2 gene silencing can increase the growth inhibition in KB/VCR cells and down regulate the cell invasion.

Key words: KB/VCR cell, cyclooxygenase-2, siRNA, proliferation, ivasion

中图分类号: 

• R739.8

• Q754