摘要： 目的：通过体外实验对新型生物吸收性多孔碳酸化羟基磷灰石（CAP）支架材料的骨传导性进行评价。方法：体外分离培养大鼠骨髓间充质干细胞，向成骨诱导后，定植于不同孔隙率及不同碳酸根含量的CAP支架材料上共同培养，通过扫描电镜、细胞黏附及增殖检测（MTT法）、碱性磷酸酶（ALP）定量检测、骨钙素（OCN）定量检测，评价成骨细胞在支架材料上的附着、增殖和分化情况。结果：成骨细胞定植于不同孔隙率及碳酸根含量的支架材料上，4 h均已开始黏附、且增殖情况良好。分化实验中，ALP和OCN在各组支架材料分化良好。不同孔隙率支架材料组间比较，40%孔隙率的实验组对ALP分化的促进作用有明显优势。结论：CAP支架材料有良好的生物相容性和骨传导性，是一种良好的组织工程支架材料。
关键词:
碳酸化羟基磷灰石,
多孔性,
骨传导性,
骨髓间充质干细胞
Abstract: Objective: To evaluate the osteoconductivity of a novel porous carbonated apitite (CAP) in vitro. Methods: Rat bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured. Well growing BMSCs were cultured with culture medium containing osteogenic inductor. Osteoblast cells obtained by osteogenic induction were seeded on the surfaces of CAP scaffolds with different porosities and carbonate contents. SEM, MTT analysis, alkaline phosphatase (ALP) activity assay, osteocalcin (OCN) activity assay were carried out to measure the osteogenic potentials of the BMSCs cells. Results: BMSCs cells could well attach to and extend on all experiment and control group samples. The alkaline phosphatase (ALP) activity assay, osteocalcin activity assay showed that BMSCs cells differentiated well on all CAP groups. And 40% NaCl content groups were more outstanding in ALP activity assay. Conclusion: The porous CAP is biocompatible and osteoconductible.
Key words:
carbonated apatite,
porosity,
osteoconductivity,
BMSCs
中图分类号:
汪安，陈建荣，于丽凤，张磊. 生物吸收性多孔碳酸化羟基磷灰石支架的骨传导性体外研究[J]. 《口腔颌面外科杂志》, 2014, 24(3): 185-.
WANG An, CHEN Jian-rong, YU Li-feng, ZHANG Lei. Osteoconductivity of Bioabsorbable Porous Carbonated Apitite Scaffolds in Vitro[J]. 《Journal of Oral and Maxillofacial Surgery》, 2014, 24(3): 185-.