摘要： 目的：研究载免疫抑制剂SB203580的纳米粒-明胶（Ms-SB203580-gelatin）纤维缓释支架对牙周膜细胞（human periodontal ligament cells，hPDLCs）的基质金属蛋白酶（matrix metalloproteninases，MMPs）表达的影响。方法：通过透析法制备载药纳米胶束（Micelles-SB203580，Ms-SB203580），并通过电纺法构建载纳米粒明胶支架，检测支架材料的细胞相容性和对牙周膜细胞MMP-2、MMP-13基因和蛋白的表达影响。结果：透射电镜显示载药纳米粒能负载于明胶纤维中；细胞与支架复合后增殖情况良好；实验组MMP-2、MMP-13的表达低于阳性对照组。结论：载药纳米粒明胶纤维缓释支架能明显地抑制炎症因子，可进一步应用于牙槽骨骨组织工程。

关键词: 免疫抑制剂, 支架, 牙周膜细胞, MMPs

Abstract: Objective: The study aimed to evaluate whether the SB203580-loaded nanoparticles-gelatin (Ms-SB203580-gelatin) scaffolds are related to reduction of  matrix metalloproteninases（MMPs） expression in human periodontal ligament cells (hPDLCs). Methods: The drug-loaded micelles（Micelles-SB203580, Ms-SB203580）was obtained by dialysis bag method and fabricate drug-loaded gelatin scaffolds by electrospining. Cell compatibility and the gene as well as protein expression of  MMP-2、MMP-13 were measured. Results: The gelatin scaffolds contain drug-loaded micelles were proved by TEM,  The hPDLCs seeded at the scaffolds demonstrated a good biocompatibility. MMP-2 and MMP-13 expression were stain slightly in the experimental group  than that of the positive group. Conclusion: The results suggest that Ms-SB203580-gelatin scaffolds have a potential to obviously reduce  inflammation and may be effective for alveolar bone repair in the future.

Key words:  immunosuppressor, scaffolds, human periodontal ligament cells, MMPs

中图分类号: 

• R782

• Q813.1