快速原型技术制备Nano-HA/PCL支架与犬骨髓基质干细胞体外复合研究

doi:10.3969/j.issn.1005-4979.2015.01.006

《口腔颌面外科杂志》 ›› 2015, Vol. 25 ›› Issue (1): 34-. doi: 10.3969/j.issn.1005-4979.2015.01.006

快速原型技术制备Nano-HA/PCL支架与犬骨髓基质干细胞体外复合研究

郭凌云，张劲娥，袁建兵，黄远亮

1. 上海市东方医院口腔科，上海 200120；2. 上海交通大学机械与动力工程学院，上海 200030

出版日期:2015-02-28 发布日期:2015-04-29
通讯作者: 黄远亮,教授. E-mail:YLHuang0115@163.com
作者简介:郭凌云(1977—)，女，硕士，山西人，主治医师.
基金资助:
上海市浦东新区卫生局优青培养计划（PWRq2011-03）

Rapid Prototyping of Nano-HA/PCL Composite Scaffolds and Osteogenic Potential of Bone Marrow Stroma Cells of Canine in vitro

GUO Ling-yun, ZHANG Jin-e, YUAN Jian-bing, HUANG Yuan-liang

1. Department of Stomatology, Shanghai Municipal Oriental Hospital, Shanghai 200120; 2. School of Mechanical Engineering, Shanghai Jiaotong University, Shanghai 200030, China

Online:2015-02-28 Published:2015-04-29

摘要/Abstract

摘要： 目的：探讨快速原型制作纳米-羟基磷灰石/聚己内酯（nano-HA/PCL）根形三维支架的细胞生物相容性，为进一步体内实验提供研究基础。方法：通过CT 扫描获得犬头颅骨影像信息，以CAD 软件实现犬下颌牙根形态的三维重建影像，应用快速原型（RP）技术制作nano-HA/PCL根形支架，经犬骨髓基质细胞接种后体外复合培养，检测支架材料的细胞相容性。结果：通过CT影像和快速原型技术，可实现解剖根形态结构nano-HA/PCL三维仿真支架。细胞支架复合培养后，扫描电镜显示细胞生长附着于支架表面；细胞与支架复合后增殖情况良好；7 d和14 d碱性磷酸酶活性高于对照组。结论：体外复合培养结果表明，RP技术制备的nano-HA/PCL支架和骨髓基质细胞生物相容性较好，可进一步应用于牙槽骨缺损骨组织工程修复动物实验。

关键词: 快速原形技术, 羟基磷灰石/聚己内酯支架, 骨髓基质细胞, 生物相容性, 犬

Abstract: Objective: To fabricate nano-hydroxyapatite/Poly-ε-caprolactone(nano-HA/PCL) scaffold with three-dimensional simulation of the root shape by using a rapid prototyping (RP) process. To evaluate biocompatibility of the scaffold used for bone tissue engineering. Methods: Parietal bone image information of canine was obtained by CT scan, three-dimensional reconstruction images were performed by CAD, roots shape nano-HA/PCL scaffolds was obtained by RP technology. Canine 3rd passage bone marrow stromal cells (BMSCs) were seeded in nano-HA/PCL scaffolds and grown in osteogenic medium. Cells were harvested after 1, 3, and 7 days. BMSCs proliferation and ALP activity were measured. Results: Scanning electron micrographs showed the BMSCs adhered to the inner surface of the scaffolds. Cell proliferation steadily increased with time, and alkaline phosphatase activity in the scaffolds at 7- day and 14- day were enhanced. Conclusion: The osteogenic potential of BMSCs seeded at the rapid prototyped nano-HA/PCL scaffolds demonstrate a good biocompatibility, and may be effective for animal experiment in alveolar bone repair in the near future.

Key words: rapid-prototyping technique, nano-hydroxyapatite/poly-ε-caprolactone（nano-HA/PCL）scaffold, bone marrow cells, biocompatibility, dogs

中图分类号:

郭凌云，张劲娥，袁建兵，黄远亮. 快速原型技术制备Nano-HA/PCL支架与犬骨髓基质干细胞体外复合研究[J]. 《口腔颌面外科杂志》, 2015, 25(1): 34-.

GUO Ling-yun, ZHANG Jin-e, YUAN Jian-bing, HUANG Yuan-liang. Rapid Prototyping of Nano-HA/PCL Composite Scaffolds and Osteogenic Potential of Bone Marrow Stroma Cells of Canine in vitro[J]. 《Journal of Oral and Maxillofacial Surgery》, 2015, 25(1): 34-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2015/V25/I1/34

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快速原型技术制备Nano-HA/PCL支架与犬骨髓基质干细胞体外复合研究

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