表面载Zn或Zn-Ag钛合金种植体骨结合比较的动物实验

doi:10.3969/j.issn.1005-4979.2018.05.007

摘要/Abstract

摘要： 目的：研究钛合金经阳极氧化改性后电化学沉积金属锌（Zn）及锌-银（Zn-Ag）复合离子种植体植入动物体内骨结合情况。方法：实验样本共分为4组：钛合金种植体酸蚀处理为A组，钛合金种植体酸蚀后阳极氧化为B组，B组基础上电化学沉积Zn膜层（C组）、Zn-Ag复合膜层（D组），每组9枚，共计36枚。分别将4组试件对称植入9只新西兰大白兔左右两侧下颌骨体，每侧2枚。于２、４、８周分批处死取骨进行CBCT、扫描电镜、元素分析、荧光标记检测。结果：钛合金经阳极氧化处理电化学沉积制备的含锌、银无机抗菌剂的金属离子膜层种植体表面微孔结构更致密；相同时间段内有膜层种植体周围骨密度均高于无膜层种植体；随时间增加Zn-Ag复合膜层种植体骨结合能力最好，周围骨矿化沉积速度及骨量更高。结论：阳极氧化处理种植体电化学加载抗菌金属离子Zn和Zn-Ag，可以赋予种植体一定抗菌性的同时具有良好生物相容性，可以提高骨结合速度，维持植入早期稳定性。

关键词: 钛合金, 阳极氧化, 锌离子, 银离子, 骨结合

Abstract: Objective: To investigate the osseointegration of anodized and Zn and Zn-Ag coated titanium alloy implants in an animals model. Methods: The experimental samples of implants were divided into four groups. In group A, titanium alloy implant was treated with acid etching. In group B, implants were coated with acid etching + anodized surface. In group C, implants were treated like group B frist, then electrochemically deposited with Zn film layer. In group D, implants were treated like group B, then group B implants coated with Zn-Ag composite film layer. 9 implants were consisted in each group, and a total of 36 implants. Four sets of test pieces were symmetrically implanted into the mandible of the left and right sides of 9 New Zealand rabbits, 2 implants were on each side. Animals were sacrificed at 2, 4, and 8 weeks for CBCT, scanning electron microscopy, elemental analysis, and fluorescent labeling examinations. Results: The surface micropore structure was denser in the metal ion membrane layer of the zinc ion-containing silver-containing inorganic antibacterial agent coated surface. The bone density around the implants in the same time points was higher than that of the non-membrane coated implants; with the addition of time, the Zn-Ag composite membrane implants had the best bone-binding ability, and the surrounding bone mineralization deposition rate and bone mass were higher than other groups. Conclusion: The anodizing treatment of the implant electrochemically loaded with antibacterial metal ions Zn and Zn-Ag can impart certain antibacterial properties to the implant while having good biocompatibility, which can effectively shorten the osseointegration cycle and maintain the early stability of implantation.

Key words: Titanium alloy, anodic oxidation, zinc ions, silver ion, osseointegration

中图分类号:

R782.4

孔毅，刘继光，张慧明，姚海涛，李慕勤. 表面载Zn或Zn-Ag钛合金种植体骨结合比较的动物实验[J]. 《口腔颌面外科杂志》, doi: 10.3969/j.issn.1005-4979.2018.05.007.

kongyi,liu ji guang, zhang hui ming,yao hai tao, li mu qin. Osseointegration to Zn- and Zn-Ag- Coated Ttanium Implants in an Animal Model[J]. 《Journal of Oral and Maxillofacial Surgery》, doi: 10.3969/j.issn.1005-4979.2018.05.007.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2018/V28/I5/272

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