颞下颌关节软骨对咬合力刺激响应特征的动物实验启示

doi:10.3969/j.issn.1005-4979.2020.02.001

《口腔颌面外科杂志》 ›› 2020, Vol. 30 ›› Issue (2): 61-66. doi: 10.3969/j.issn.1005-4979.2020.02.001

• 专家论坛 • 下一篇

颞下颌关节软骨对咬合力刺激响应特征的动物实验启示

王美青(), 杨鸿旭, 张虹云, 刘倩

第四军医大学口腔医学院颞下颌关节病科，陕西西安 710032

收稿日期:2019-12-19 修回日期:2020-01-15 出版日期:2020-04-28 发布日期:2020-04-24
通讯作者: 王美青，教授. E-mail: mqwang@fmmu.edu.cn

作者简介:

王美青（1962—），女，山东章丘人，第四军医大学口腔医学院口腔解剖生理学教研室及颞下颌关节病科教授、主任医师。The Journal of Bone and Mineral Research、Journal of Dental Research(2015—2018)、Journal of Oral Rehabilitation、Archives of Oral Biology和Cranio-the Journal of Craniomandibular Practice等国际杂志，以及《中华口腔医学杂志》《华西口腔医学杂志》《上海口腔医学》《中国实用口腔科杂志》《中华口腔医学研究杂志》《今日口腔》《转化医学》等国内期刊编委。主编《口腔解剖生理学》（第7版）、《𬌗学》（第4版）等卫生部高等院校规划教材。曾获全军教学成果二等奖，军队育才银奖、金奖，全军优秀教师称号。以第一负责人主持11项国家自然科学基金项目（1993—2020）,在国际口腔医学SCI收录的杂志上以第一作者或通信作者发表研究论文70余篇，主编出版专著4部。曾主持完成全军科技进步二等奖1项和陕西省科技进步一等奖1项。自2014年有记载以来一直是中国内地艾斯维尔（Elsevier）出版物高被引学者。通过近20年的探索，以流行病学研究、电生理检测数据、影像诊断资料以及动物模型为代表的研究成果，在国际上率先提出并初步证明了异常咬合可以作为独立的致病因素，导致颞下颌关节骨关节病样改变。

基金资助:
国家自然科学基金重点国际合作项目(81920108013)

Responses of the Temporomandibular Joint Cartilage to Occlusion Alterations: the Obtaining from Animal Investigation

WANG Meiqing(), YANG Hongxu, ZHANG Hongyun, LIU Qian

Department of Orofacial Pain and Temporomandibular Joint, School and Hospital of Stomatology, the Fourth Military Medical University, Xian 710032, Shaanxi Province, China

Received:2019-12-19 Revised:2020-01-15 Online:2020-04-28 Published:2020-04-24

摘要/Abstract

摘要：

关节软骨主要的功能之一是承受一定的运动负荷，颞下颌关节髁突软骨所承受的功能负荷与咬合密切相关，因而颞下颌关节髁突软骨可以随咬合的变化而进行相应的改建。我们利用大鼠、小鼠进行的实验研究显示，用佩戴金属冠的方法加高小鼠双侧前牙咬合（bilateral anterior elevation，BAE），可导致小鼠髁突软骨明显增厚；而用金属冠制作单侧前牙反𬌗（unilateral anterior crossbite, UAC）不良修复体，则可导致髁突软骨出现明显的退行性变，直至髁突变形。我们利用这些可导致髁突软骨出现增殖性或退行性改建的动物模型进行在体基因修饰实验，探索髁突软骨改建过程中与细胞自噬、凋亡、终末分化等活动密切相关的信号通路，例如哺乳动物雷帕霉素靶蛋白（mammalian target of rapmycin,mTOR）信号，印第安刺猬信号（India hedgehog, Ihh），以及甲状旁腺素及其相关蛋白受体信号等，其结论有望指导临床工作中在纠正异常咬合基础上，从细胞和分子水平干预软骨细胞功能，预防和治疗颞下颌关节软骨退变。

关键词: 颞下颌关节, 咬合, 动物实验, 骨关节炎, 软骨

Abstract:

One of the main functions of the articular cartilage is to bear loads during various joint movements. The loads suffered by the temporomandibular joint (TMJ) are linked with occlusion. The mandibular condylar cartilage remodels in response to dental occlusion alterations. Through applying metal tubes onto incisors of rodents, we had reported that the bilateral anterior elevation (BAE) bite increases the condylar cartilage thickness, while unilateral anterior crossbite (UAC) relation leads to degenerative remodeling in the mandibular condylar cartilage which ends in deformation of the condyles. Through application of these dental stimulations, BAE and UAC, onto the mice with different genetic mutations, we investigated the signaling pathways such as mammalian target of rapamycin (mTOR), Indian hedgehog (Ihh), and parathyroid hormone receptor-1 (PTHR1) which involve in chondrocytes autophagy, apoptosis and cellular terminal differentiation. The obtaining will bring a new insight in treatment of TMJ osteoarthritis, that is, dental occlusion correct in combined with molecular and cellular interventions.

Key words: temporomandibular joint, occlusion, animal experiment, osteoarthritis, cartilage

中图分类号:

R782.6

王美青, 杨鸿旭, 张虹云, 刘倩. 颞下颌关节软骨对咬合力刺激响应特征的动物实验启示[J]. 《口腔颌面外科杂志》, 2020, 30(2): 61-66.

WANG Meiqing, YANG Hongxu, ZHANG Hongyun, LIU Qian. Responses of the Temporomandibular Joint Cartilage to Occlusion Alterations: the Obtaining from Animal Investigation[J]. 《Journal of Oral and Maxillofacial Surgery》, 2020, 30(2): 61-66.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2020/V30/I2/61

图/表 1

图1 小鼠髁突软骨对双侧前牙加高（BAE）和单侧前牙反𬌗（UAC）的响应表现（×200）注：A.对照组HE染色结果；B.对照组SO染色结果；C.对照组VK染色结果；D.BAE组HE染色结果；E. BAE组SO染色结果；F. BAE组VK染色结果；G.UAC组HE染色结果；H. UAC组SO染色结果；I. UAC组VK染色结果。BAE组及UAC组处置时，鼠龄为6周龄，取材时间为施加BAE或UAC后7周时。HE染色：苏木精-伊红染色；SO染色：番红O染色；VK染色：冯库萨染色。

Figure 1 Responses of the mouse condylar cartilage to bilateral anterior elevation (BAE) and unilateral anterior crossbite (UAC)（×200）

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