舌鳞癌组织中CHK1和RAD51的表达及其临床病理意义

doi:10.3969/j.issn.1005-4979.2012.06.004

摘要/Abstract

摘要： 目的：研究舌鳞状细胞癌和癌旁正常上皮组织中，细胞周期检测点激酶1（checkpoint kinase 1, CHK1）和DNA双链断裂修复蛋白（RAD51）的表达水平及其临床病理意义。方法：45例舌鳞状细胞癌和10例癌旁正常上皮组织常规制作石蜡包埋切片，CHK1和RAD51染色方法为EnVisionTM免疫组织化学法；应用统计软件SPSS 13.0行χ２检验。结果：舌鳞状细胞癌中CHK1和RAD51表达阳性率明显高于癌旁正常上皮（χ２=3.67, P=0.05 ）。组织学分级I～II级、临床分期I～II期及无颈部淋巴结转移的病例，其CHK1和RAD51表达阳性率，明显低于组织学分级III～IV级、临床分期III～IV期及颈部淋巴结转移的病例（P＜0.05）。结论：CHK1和RAD51的表达水平，对舌鳞状细胞癌的发生、进展、临床生物学行为等方面可能有重要影响，CHK1和（或）RAD51阳性表达者预后不良。

关键词: 舌鳞状细胞癌, 细胞周期检测点激酶（CHK), DNA双链修复蛋白（RAD51）, 免疫组织化学, 预后

Abstract: Objective: To study the expressive levels of CHK1 and RAD51 in their peritumoral normal tissues or the squamous cell cancer in tongue, and their clinic pathological significances. Methods: EnvisionTM immunohistochemistry was used to detect the levels of CHK1 and RAD51 in conventional paraffin-embedded sections from specimens of squamous cell cancer (n=45) and peritumoral normal tissues (n=10). The SPSS13.0 program was used to calculate interrelationships between CHK1 and RAD51 and histological or clinical factors by χ2 independence test. Results: Expression intensity of CHK1 and RAD51 were significantly higher in squamous cell cancer than those in peritumoral normal tissues. CHK1 vs RAD 51 was 53.3% vs 20.0% (χ2=3.67, P=0.05). The positive rates of CHK1 and RAD51 expression were significantly lower in the cases of histological grade I-II, clinical stage I-II, and no-metastasis of neck lymphnode cases, compared with those in the histological grade III-IV, clinical stage III-IV, and metastasis of neck lymphnode cancer(P<0.05). Conclusion：The expressive levels of CHK1 and/or RAD51 might importantly affect on their carcinogenesis, progression and biologic behaviors of tongue cancer. Over-expression of CHK1 and/or RAD51 might have poorly prognosis.

Key words: tongue squamous cell carcinoma, checkpoint kinase 1(CHK1), RAD51, immunohistocytochemistry, prognosis

中图分类号:

R739.8

邓年丰，冯云枝. 舌鳞癌组织中CHK1和RAD51的表达及其临床病理意义[J]. 《口腔颌面外科杂志》, 2012, 22(6): 393-396.

DENG Nian-feng, FENG Yun-zhi. Expression of CHK1 and RAD51 and its Clinic Pathological Significances in the Tongue Squamous Cell Carcinoma[J]. 《Journal of Oral and Maxillofacial Surgery》, 2012, 22(6): 393-396.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2012/V22/I6/393

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