偏颌大鼠模型的构建及其髁突CT影像和组织学评价

doi:10.3969/j.issn.1005-4979.2021.05.003

《口腔颌面外科杂志》 ›› 2021, Vol. 31 ›› Issue (5): 278-284. doi: 10.3969/j.issn.1005-4979.2021.05.003

偏颌大鼠模型的构建及其髁突CT影像和组织学评价

张月姣¹(), 徐小杰², 刘倩³, 赵雅莉⁴, 刘继光¹(), 王美青¹^,³()

¹ 佳木斯大学口腔医学院，黑龙江佳木斯 154007
² 新乡医学院第三附属医院，河南新乡 453003
³ 空军军医大学第三附属医院口腔解剖生理学教研室，陕西西安 710032
⁴ 解放军总医院第五医学中心肝胆外科一中心，北京 100039

收稿日期:2020-12-01 修回日期:2021-03-31 出版日期:2024-03-21 发布日期:2021-12-30
通讯作者: 刘继光, 王美青
作者简介:
张月姣（1994—），女，河北人，硕士.E-mail: 1064382106@qq.com
基金资助:
国家自然科学基金(81920108013)

Construction of the mandible deviation occlusion rat model and micro-CT radiography and histology evaluation of the mandibular condyles

ZHANG Yuejiao¹(), XU Xiaojie², LIU Qian³, ZHAO Yali⁴, LIU Jiguang¹(), WANG Meiqing¹^,³()

¹ School of Stomatology, Jiamusi University, Jiamusi 154007, Heilongjiang Province
² The Third Hospital, Xinxiang Medical Collage, Xinxiang 453003, Henan Province
³ Department of Oral Anatomy and Physiology, Third Affiliated Hospital, Air Force Medical University, Xi'an 710032, Shaanxi Province
⁴ First Center of Hepatobiliary Surgery, the Fifth Medical Center, PLA General Hospital, Beijing 100039, China

Received:2020-12-01 Revised:2021-03-31 Online:2024-03-21 Published:2021-12-30
Contact: LIU Jiguang, WANG Meiqing

摘要/Abstract

摘要：

目的： 构建下颌偏斜大鼠模型，观察该实验性偏颌大鼠髁突显微CT（micro-CT）影像及其组织学变化。方法： 选取12只6周龄雌性SD大鼠，将其随机等量分为对照组和偏颌组，每组6只。偏颌造模方法：将上颌左侧中切牙及下颌双侧中切牙分别粘接金属套筒冠不良修复体，上颌金属套筒冠制备唇向斜面导板，使上下颌切牙有正常方向的覆牙合覆盖关系，下颌金属套筒冠制备与牙长轴呈45°角的近远中向斜面导板，使动物咬合时导板可以引导下颌向右。对照组不做任何处理。2组大鼠饲养12周后取材，对双侧髁突进行micro-CT检测及组织学染色。将对照组双侧髁突测量的数据合并取平均值，将偏颌组分为偏颌组-左侧和偏颌组-右侧，分别与对照组比较。结果： Micro-CT结果显示，对照组大鼠髁突关节面光滑、完整，骨质致密。与对照组髁突相比，偏颌组髁突长度和宽度出现不同程度的增加，但骨小梁有明显的吸收；12个髁突中有3个左侧髁突表面出现陷窝。组织学染色结果显示，对照组大鼠髁突软骨细胞层次分明，而偏颌组大鼠左侧髁突骨性陷窝内有软骨组织或纤维组织充填。组织测量学结果显示，偏颌组双侧大鼠髁突未矿化软骨的中带较对照组有显著增厚（P<0.05），偏颌组左侧髁突未矿化软骨的后带较对照组有明显增厚（P<0.05）。结论： 实验性偏颌可致大鼠双侧髁突骨和软骨出现非对称性异常改建，偏颌对侧髁突表面可出现局部纤维软骨样组织增厚，在影像学上表现为关节面上缺损样陷窝。

关键词: 颞下颌关节, 骨吸收, 偏颌, 软骨陷窝

Abstract:

Objective: To construction a mandible deviated occlusion(MDO) rat model and investigate the changes in the micro-CT images and histology of condyles. Methods: Twelve 6-week-old female SD rats were randomly divided into control group and MDO group (n=6 in each group). The method of MDO model: The left maxillary central incisors and the bilateral mandibular central incisors were bonded with the metal tubed aberrant prosthesis. The maxillary metal tube was designed to have a lingual inclined guide plate in order to achieve normally derived over-jet and over-bite relationship. The mandibular metal tube was designed to have an inclined plate, 45 degrees in mesial-distal direction to guide the mandible to deviate to right side when rat biting. The control group did not do any treatment. The rats in the two groups were fed for 12 weeks, and bilateral mandibular condyles were collected for micro-CT scanning and then taken for histological staining. The data of bilateral condyles in the control group were combined to get the average value. The data from the MDO group were divided into the MDO-left group and the MDO-right group. Results: Micro-CT images showed that the condyles in control group had smooth and intact articular surface. The density of the subchondral bone was normally impact. In contrast, the length and width of the condyles in MDO group were increased in varying degree and trabecular bone loss was obviously absorbed. Three left condyles out of the total twelve condyles in MDO group showed lacunae on the articular surface. Histological staining showed that the condyle chondrocytes in control group had well organized zonal cartilage. While in the MDO-left group, lacunaes of left condyles were filled with cartilage tissue or fibrous tissue. Histomorphometry analysis showed that the cartilage in middle third of the condyle in both MDO-left and MDO-right groups was thicker than that in control group (P<0.05). Unmineralized cartilage in posterior third of MDO-left group was thicker than that in control group (P<0.05). Conclusion: MDO could lead to asymmetrically aberrant remodeling of bone and cartilage in the mandibular condyles, and formation of cartilage projections in the articular surface which displayed on micro-CT images as lacuna of the condylar articular surface.

Key words: temporomandibular joint, trabecular bone resorption, mandible deviation occlusion, cartilage lacunae

张月姣, 徐小杰, 刘倩, 赵雅莉, 刘继光, 王美青. 偏颌大鼠模型的构建及其髁突CT影像和组织学评价[J]. 《口腔颌面外科杂志》, 2021, 31(5): 278-284.

ZHANG Yuejiao, XU Xiaojie, LIU Qian, ZHAO Yali, LIU Jiguang, WANG Meiqing. Construction of the mandible deviation occlusion rat model and micro-CT radiography and histology evaluation of the mandibular condyles[J]. 《Journal of Oral and Maxillofacial Surgery》, 2021, 31(5): 278-284.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2021/V31/I5/278

图/表 7

图1 对照组大鼠及偏颌造模后大鼠的咬合正面照及其示意图 A. 对照组大鼠前牙正面观；B. 偏颌组大鼠前牙模型正面观；C. 对照组大鼠前牙示意图；D. 偏颌组大鼠前牙模型示意图。偏颌组动物的下颌在戴有金属套管的切牙咬合引导下向右偏斜。

Figure 1 Representative frontal view and the sketch map of the incisal occlusal relationship in control group and MDO group of rats

图2 髁突micro-CT影像测量方法蓝色箭头指示髁突长度测量标志点；绿色箭头指示髁突宽度测量标志点；黄色立方体为髁突软骨下骨测量选区，箭头右侧的正方体为左侧黄色立方体选区的局部放大图。

Figure 2 The measuring methods of micro-CT images

图3 冯库萨染色片和甲苯胺蓝染色片上髁突前、中、后分区及其软骨厚度 A. 颞下颌关节髁突冯库萨染色，髁突前、中、后分区（×25）；B. 颞下颌关节髁突甲苯胺蓝染色，髁突前、中、后分区（×25）；C. 冯库萨染色法测量未矿化软骨厚度（×200）；D. 甲苯胺蓝染色法测量软骨体部厚度（×200）。

Figure 3 The cartilage thickness revealed by sections with VK staining and TB staining, the trisections were the anterior, middle and posterior third of the condyle

图4 对照组与偏颌组-左侧及偏颌组-右侧髁突micro-CT检测指标比较 A. 髁突长度; B. 髁突宽度; C. BMD; D. BV/TV; E. BS/BV; F. Tb.Th; G. Tb.N; H. Tb.Sp。 *表示P<0.05，与对照组比较；**表示P<0.01，与对照组比较；***表示P<0.001，与对照组比较。

Figure 4 Comparisons of the parameters measured from the micro-CT images between the control group and the left and right side condyles from the MDO group

图5 对照组、偏颌组-左侧和偏颌组-右侧大鼠髁突矢状面CT图片 A. 对照组大鼠髁突矢状面CT图片；B. 偏颌组-左侧髁突矢状面CT图片，箭头所示为骨性关节面的凹陷；C. 偏颌组-右侧髁突矢状面CT图片。

Figure 5 The representative micro-CT images in the sagittal sections of the mandibular condyles from the control group and that of the left and right side condyles from the MDO group

图6 对照组及偏颌组-左侧和偏颌组-右侧髁突冯库萨染色、甲苯胺蓝染色及苏木精-伊红染色图

Figure 6 Representative images with VK staining, TB staining and HE staining for the sagittal sections of the mandibular condyles from the control group and that of the left and right side condyles from the MDO group

图7 对照组与偏颌组-左侧及偏颌组-右侧髁突冯库萨染色图片显示的未矿化软骨厚度及甲苯胺蓝染色图片显示的软骨体部厚度（选区见图3）测量结果比较 A~C. 3组未矿化软骨厚度；D~F. 3组软骨体部厚度。*表示P<0.05,与对照组比较；**表示P<0.01,与对照组比较。

Figure 7 Comparisons of the cartilage body thickness (based on TB staining) and the un-mineralized cartilage thickness (based on VK staining) between the control group and the left and right side condyles from the MDO group

参考文献 17

[1]	殷晓丽, 刘洋, 王军. 伴发于偏颌畸形的颞下颌关节内部结构变化[J]. 国际口腔医学杂志, 2020, 47(5): 567-573.
[2]	Chappard C, Peyrin F, Bonnassie A, et al. Subchondral bone micro-architectural alterations in osteoarthritis: A synchrotron micro-computed tomography study[J]. Osteoar-thritis Cartilage, 2006, 14(3): 215-223.
[3]	Kawamoto T, Kawamoto K. Preparation of thin frozen sections from nonfixed and undecalcified hard tissues using Kawamoto′s film method(2020)[J]. Methods Mol Biol, 2021, 2230: 259-281.
[4]	Zhang M, Wang H, Zhang J, et al. Unilateral anterior crossbite induces aberrant mineral deposition in degenerative temporomandibular cartilage in rats[J]. Osteoarthritis Cartilage, 2016, 24(5): 921-931. doi: 10.1016/j.joca.2015.12.009 URL
[5]	王美青, 杨鸿旭, 张虹云, 等. 颞下颌关节软骨对咬合力刺激响应特征的动物实验启示[J]. 口腔颌面外科杂志, 2020, 30(2): 61-66. doi: 10.3969/j.issn.1005-4979.2020.02.001
[6]	向喜林, 陈仪, 戴其昌. 不良咬合与颞下颌关节紊乱综合症发病的生物力学分析[J]. 医用生物力学, 1998, 13(1): 21-24.
[7]	陈丹鹏, 史宗道, 易新竹, 等. 前牙及后牙反合与颞下颌关节紊乱症关系的流行病学研究[J]. 口腔正畸学杂志, 1994, 1(1): 10-12.
[8]	赵晓敏, 包旭英, 龚忠诚, 等. 新疆大学生颞下颌关节紊乱病相关因素的logistic回归分析[J]. 口腔医学, 2015, 35(10): 874-877,880.
[9]	张渊, 王美青, 凌伟. 咬合接触对颞下颌关节生物力学影响的有限元研究[J]. 医用生物力学, 2005, 20(3): 163-166,170.
[10]	Zhang M, Yang H, Wan X, et al. Prevention of injury-induced osteoarthritis in rodent temporomandibular joint by targeting chondrocyte CaSR[J]. J Bone Miner Res, 2019, 34(4): 726-738. doi: 10.1002/jbmr.3643 pmid: 30496623
[11]	Liu Q, Yang HX, Duan J, et al. Bilateral anterior elevation prosthesis boosts chondrocytes proliferation in mice mandibular condyle[J]. Oral Dis, 2019, 25(6): 1589-1599. doi: 10.1111/odi.13128 pmid: 31132303
[12]	Feng C, Ji P, Luo P, et al. Estrogen-mediated MicroRNA-101-3p expression represses hyaluronan synthase 2 in synovial fibroblasts from idiopathic condylar resorption patients[J]. J Oral Maxillofac Surg, 2019, 77(8): 1582-1593. doi: 10.1016/j.joms.2019.02.032 URL
[13]	He YF, Lin H, Lin QP, et al. Morphologic changes in idiopathic condylar resorption with different degrees of bone loss[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2019, 128(3): 332-340. doi: 10.1016/j.oooo.2019.05.013 URL
[14]	Kristensen KD, Schmidt B, Stoustrup P, et al. Idiopathic condylar resorptions: 3-dimensional condylar bony deformation, signs and symptoms[J]. Am J Orthod Dentofacial Orthop, 2017, 152(2): 214-223. doi: 10.1016/j.ajodo.2016.12.020 URL
[15]	王启明, 方泽强, 冯驰. 偏侧咀嚼与颞下颌关节紊乱病的相关性研究[J]. 激光杂志, 2014, 35(5): 85-87.
[16]	李俊慧, 黄银莉. 偏侧咀嚼对青少年颞下颌关节形态的影响[J]. 宁夏医学杂志, 2019, 41(5): 442-444.
[17]	倪峰. 骨性偏颌患者颞下颌关节应力分布的分析研究[D]. 西安: 第四军医大学, 2012.

选择文件类型/文献管理软件名称

选择包含的内容

偏颌大鼠模型的构建及其髁突CT影像和组织学评价

Construction of the mandible deviation occlusion rat model and micro-CT radiography and histology evaluation of the mandibular condyles

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 17

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨春丽, 骆洋, 刘雯华, 林萤, 王荣, 李新. 数字化辅助稳定咬合导板在治疗颞下颌关节紊乱病中的应用[J]. 《口腔颌面外科杂志》, 2022, 32(5): 292-296.
[2]	董洋, 陈广庶, 干春凤, 张壮. 颞下颌关节骨关节炎患者血清IL-1β、OPN、TGF-β1水平变化及其临床意义的研究[J]. 《口腔颌面外科杂志》, 2022, 32(4): 225-230.
[3]	沈达, 李健. 可吸收带线锚固钉与金属锚固钉治疗颞下颌关节盘不可复性前移位的短期疗效比较[J]. 《口腔颌面外科杂志》, 2022, 32(1): 55-59.
[4]	李涛, 黎松龄, 周燕平. 自体富血小板血浆关节腔注射治疗对不同年龄段颞下颌关节骨关节炎患者的疗效评价[J]. 《口腔颌面外科杂志》, 2021, 31(6): 355-359.
[5]	钟阳, 满城. 颞下颌关节骨关节炎发病机制的研究进展[J]. 《口腔颌面外科杂志》, 2021, 31(4): 245-248.
[6]	胡佳慧, 王海丞, 黄洁, 周笑天, 王佐林. 种植体间距与种植体周炎关系的实验研究[J]. 《口腔颌面外科杂志》, 2021, 31(3): 142-149.
[7]	黄洁, 王海丞, 胡佳慧, 周笑天, 王佐林. 种植体颈部与唇侧骨板间距离对唇侧牙槽骨吸收及牙龈退缩的影响[J]. 《口腔颌面外科杂志》, 2021, 31(2): 75-80.
[8]	李艳艳, 冯亚平, 柯金, 龙星. NLRP3和Caspase-1在颞下颌关节骨关节炎滑膜中的表达[J]. 《口腔颌面外科杂志》, 2021, 31(2): 81-84.
[9]	叶莉娜, 何家才. 单侧后牙正锁𬌗、反𬌗对下颌升支及髁突对称性影响的临床观察[J]. 《口腔颌面外科杂志》, 2020, 30(6): 382-386.
[10]	王美青，杨鸿旭，张虹云，刘倩. 颞下颌关节软骨对咬合力刺激响应特征的动物实验启示[J]. 《口腔颌面外科杂志》, 2020, 30(2): 61-.
[11]	郑枭，满城，郑德鑫，江海涛，程娇. 罕见右侧颞下颌关节骨性强直伴同侧颌面部广泛异位骨化1例及文献复习[J]. 《口腔颌面外科杂志》, 2020, 30(2): 121-.
[12]	王晓瑞1, 高燕2, 高菊荣1, 刘玉红1. 磨牙症患者颞下颌关节结构的锥形束CT测量分析研究[J]. 《口腔颌面外科杂志》, 2019, 29(2): 84-87.
[13]	周洁, 姜蕾, 赵云富. 一种国产牙种植体5年临床效果评价[J]. 《口腔颌面外科杂志》, 2018, 28(6): 327-331.
[14]	曲陶然, 屠逸琳, 苏俭生. 嗜神经病毒示踪成年小鼠开颌反射神经环路的实验研究[J]. 《口腔颌面外科杂志》, 2018, 28(5): 252-257.
[15]	王珊，胡娟，胡阿特·哈德尔，买买提吐逊·吐尔地. 黏弹补充法联合咬合板治疗聂颌关节不可复性盘前移疗效分析[J]. 《口腔颌面外科杂志》, 2018, 28(4): 209-214.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

偏颌大鼠模型的构建及其髁突CT影像和组织学评价

Construction of the mandible deviation occlusion rat model and micro-CT radiography and histology evaluation of the mandibular condyles

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 17

相关文章 15

编辑推荐

Metrics

本文评价