摘要： 目的：研究双膦酸盐（BPs）和地塞米松联合应用对颌骨和颧骨创伤后骨改建的影响。方法：60只8～12周龄雌性C57BL6小鼠，每周2次腹腔注射唑来膦酸和地塞米松，以腹腔注射缓冲液为对照组。1周后将小鼠右上颌第一磨牙拔除，右侧颧骨制造骨创伤，后继续给药分别至术后第1天、第3周和第8周。小鼠安乐死后，取颌骨和颧骨，经不同处理后进行Micro?鄄CT扫描、HE染色、免疫组织化学染色、质谱?鄄色谱联用检测。结果：经双膦酸盐联合地塞米松处理后，颌骨和颧骨的骨改建均被抑制，出现骨细胞凋亡、辐射不透性死骨形成、炎症反应、骨小梁形态紊乱等现象，同时颌骨沉积的唑来膦酸的含量明显高于颧骨（P<0.05）。结论：双膦酸盐和地塞米松联合应用，可引起小鼠颌骨和颧骨创伤后的骨改建被抑制。

关键词: 双膦酸盐；  , 骨改建；  , 骨坏死；  , 颌骨；  , 小鼠

Abstract: Objective: To evaluate the impact of bisphosphonates and dexamethasone on the bone remodeling process of maxilla and cheekbone after injury.   Methods: 60 female wild-type C57BL6 mice aged 8-10 weeks were split into two groups. The right first maxillary molar was extracted and an artificial invasive bone nest was made in the cheekbone of each mice. The experimental group was treated with zoledronate and dexamethasone intraperitoneally, while the control group received only PBS till the different time points of 1 day, 3 weeks and 8 weeks respectively after trauma. After euthanized, properties of the injured sites were assessed using micro-computed tomography(micro-CT), histopathology  and immunohistochemistry analysis, and high performance liquid chromatography-mass spectrometric(LC-MS) methods.  Results: Suppression of bone remodeling occurred both in the maxilla and cheekbone after injury, including empty osteocyte lacunae, sequestra, inflammation, radiopaque alveolar bone and irregular trabeculae. The zoledronate content absorbed by maxilla was much higher than that of cheekbone(P<0.05).  Conclusions: The bone remodeling processes of maxilla and cheekbone after injury were both suppressed by bisphosphonates and dexamethasone.

Key words: bisphosphonates, bone remodeling, osteonecrosis, jaws, mice

中图分类号: 

• R782.4