摘要： 目的：观察脱细胞真皮基质（acellular dermal matrix, ADM）作为引导组织再生（guided tissue regeneration, GTR）屏障膜在治疗Ⅱ度根分叉缺损时的牙周组织再生情况。方法：在犬的两侧下颌第三、四前磨牙制造Ⅱ度根分叉缺损模型，将ADM作为GTR屏障膜覆盖在根分叉缺损区表面，于术后8周观察和测量根分叉处牙周组织的再生情况，并与空白对照组作比较。结果：术后8周，ADM组和空白对照组的临床附着丧失（clinical attachment loss, CAL）平均分别为1.90 mm和2.85 mm，差异有统计学意义（P＜0.05）；ADM组的新骨面积、新骨高度、新生牙骨质高度分别为8.23 mm2、4.52 mm、4.72 mm，明显大于对照组的1.75 mm2、0.91 mm、0.94 mm，而上皮和结缔组织面积则小于对照组，分别为0.02、0.54 mm2和0.10、5.56 mm2，差异均有统计学意义（P＜0.05）。结论：ADM作为GTR屏障膜治疗下颌Ⅱ度根分叉缺损，能比空白对照组获得更多的临床附着和再生牙周组织。
关键词:
脱细胞真皮基质； ,
引导组织再生； ,
屏障膜； ,
Ⅱ度根分叉缺损； ,
犬
Abstract: Objective: To observe the short-term effects of ADM as guided tissue regeneration (GTR) barrier membrane on periodontal regeneration in the treatment of class Ⅱ furcation defects. Methods: Experimental groups were comprised of 4 adult male beagle dogs. Class Ⅱ furcation defects were surgically produced on the buccal aspects of the mandibular 3rd and 4th premolars of both sides in dogs. GTR therapy with ADM was performed on one quadrant (ADM group); while no barrier membrane was used on the contralateral quadrant (control group). 8 weeks after reconstructive therapy, descriptive histological analysis and histomorphometric measurements of the newly formed tissues in the healed furcation sites were evaluated. Results: At 8 weeks, the mean clinical attachment loss were 1.90 mm and 2.85 mm for the ADM and control groups respectively. There was statistically significant differences between the two groups (P＜0.05). The ADM group showed significant new bone formation and new cementum, and less formation of epithelium and connective tissue as compared to the control group. The new bone area was 8.23 mm2 for the ADM group and 1.75 mm2 for the control group (P＜0.05). The new bone height was 4.52 mm for the ADM group and 0.91 mm for the control group (P＜0.05). The new cementum height was 4.72 mm for the ADM group and 0.94 mm for the control group (P＜0.05). The epithelium tissue area was 0.02 mm2 for the ADM group and 0.10 mm2 for the control group (P＜0.05). The connective tissue area was 0.54 mm2 for the ADM group and 5.56 mm2 for the control group (P＜0.05). Conclusion: ADM demonstrated more gain in clinical attachment and greater increase in regenerative periodontal tissues when used as GTR barrier membrane in Class Ⅱ furcation defects.
Key words:
acellular dermal matrix,
guided tissue regeneration,
barrier membrane,
Class Ⅱ furcation defect； dog
中图分类号:
王乾锋1， 刘宏伟2. 脱细胞真皮基质作为GTR屏障膜治疗Ⅱ度根分叉缺损的实验研究[J]. 《口腔颌面外科杂志》, 2013, 23(5): 347-351.
WANG Qian-feng1, LIU Hong-wei2. Acellular Dermal Matrix as GTR Barrier Membrane on Periodontal Regeneration in the Treatment of Class Ⅱ Furcation Defects[J]. 《Journal of Oral and Maxillofacial Surgery》, 2013, 23(5): 347-351.