摘要： 目的：联合检测人涎腺腺样囊性癌组织中MMP-9、CD147的表达并分析其相关性，探讨其与临床病理参数间的关系，为腺样囊性癌的临床治疗和预后判断提供理论依据。方法：选择山西医科大学第一医院病理科存档的腺样囊性癌组织标本21例（癌组），正常涎腺组织6例（对照组）。21例腺样囊性癌分别依据临床分期、有无侵犯神经进行分组。运用免疫组织化学PV-9000二步法检测MMP-9、CD147，结果用SPSS 13.0软件分析。结果：MMP-9及CD147在腺样囊性癌组中的阳性表达率（分别为76.2%和81.0%）明显高于在对照组中的阳性表达率（分别为16.7%和16.7%），差异有统计学意义（分别为P<0.05和P<0.01）。Ⅲ+Ⅳ期腺样囊性癌病例MMP-9及CD147的阳性表达率（均为100.0% ），明显高于Ⅰ+Ⅱ期病例（分别为44.4%和55.6%），差异有统计学意义（分别为P<0.01和P<0.05）。 有无侵犯神经的病例组间比较，MMP-9及CD147的表达差异没有统计学意义（P>0.05）。MMP-9和CD147在腺样囊性癌组织中均呈阴性、弱阳性、阳性、强阳性表达者分别为4、5、7和3例，表达一致率为90.5%，Kappa值为0.870。结论：MMP-9和CD147均可作为反映腺样囊性癌细胞生物学行为的客观参考指标，其表达与临床分期密切相关，且二者之间的表达有正相关。

关键词: 涎腺, 腺样囊性癌, 免疫组织化学, MMP-9, CD147

Abstract: Objective: To investigate the expression of matrix metalloproteinase (MMP)-9 and CD147 in human salivary adenoid cystic carcinoma (SACC) tissues and their correlations. Methods:  21 cases of human SACC tissues  and  6 cases of normal human salivary tissues  were  examined by immunohistochemical method. The relationship between the expression and clinical-pathological behaviors was also analyzed. Follow-up data were statistically analyzed. Results: The expression of MMP-9 and CD147 in SACC tissues was higher than those in normal salivary tissues. Positive expression rate of MMP-9 and CD147 in SACC tissues was 76.2% and 81.0% respectively, significantly higher than those of normal salivary tissues (P<0.05, P<0.01 respectively). The staining intensity of MMP-9 and CD147 in SACC tissues was closely associated with the clinicopathologic stage (P<0.01, P<0.05 respectively), but no distinct difference was found in other clinical parameters. There was also a significant correlation in staining intensity between MMP-9 and CD147. The co-expression rate of MMP-9 and CD147 in SACC was 90.5%. Conclusion: (1) MMP-9 and CD147 may be an  objective marker  to estimate the behaviors of SACC. (2)The expression of MMP-9 and CD147 in SACC tissues correlates with clinical stage. (3) There is a significant positive correlation between the expression of MMP-9 and CD147.

Key words: salivary gland, adenoid cystic carcinoma, immunohistochemistry, matrix metalloproteinase-9（MMP-9）, CD147

中图分类号: 

• R739.87