显微CT与硬组织切片技术在评价牙种植体骨结合中应用的对比研究

doi:10.3969/j.issn.1005-4979.2016.03.009

《口腔颌面外科杂志》 ›› 2016, Vol. 26 ›› Issue (3): 195-201. doi: 10.3969/j.issn.1005-4979.2016.03.009

显微CT与硬组织切片技术在评价牙种植体骨结合中应用的对比研究

赖春花，周磊，徐淑兰，郭泽鸿，岳新新

广东省口腔医院种植中心，广东广州 510280

收稿日期:2015-12-29 修回日期:2016-02-23 出版日期:2016-06-28 发布日期:2016-08-08
通讯作者: 周磊，教授. E-mail:zho668@263.net
作者简介:赖春花（1983—），女，广东人，主治医师，博士. E-mail: gdmclch@163.com
基金资助:
国家自然科学基金（81170998）；广东省医学科研基金（C2012034）

A Comparative Study of micro-CT and Hard Tissue Section Technique in the Evaluation of Osseointegration around Dental Implants

LAI Chun-hua, ZHOU Lei, XU Shu-lan, GUO Ze-hong, YUE Xin-xin

Department of Dental Implant, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China

Received:2015-12-29 Revised:2016-02-23 Online:2016-06-28 Published:2016-08-08

摘要/Abstract

摘要： 目的：探讨最佳反映牙种植体表面骨结合情况的检测方法。方法：4只beagle犬下颌骨延期植入40枚种植体，在2周和4周时取样进行显微CT检测和硬组织切片制作，对比两种检测方法的效果和特点。结果：显微CT具有连续性和完整性的优势，可以宏观地观察到种植体周围骨质情况，但是由于金属伪影的存在，它对种植体-骨界面成骨现象显示不够清晰。硬组织切片是一种单层组织切片，制作过程较复杂，但其对种植体-骨界面的细微情况显示较清晰。结论：显微CT更适用于对种植体周围总体成骨的评估，而种植体表面成骨的细微变化宜采用硬组织切片。

关键词: 骨结合, , , 钛种植体, , , 硬组织切片, , , 显微CT

Abstract: Objective: To compare the efficiency of two methods in detecting the osseointegration on titanium implant surface. Methods: Four beagle dogs were installed with a total of 40 delayed implants. At 2-week and 4-week after implantation, Micro CT detection and hard tissue slices analysis were performed to evaluate the osteointegration characteristics and differences. Results: The Micro CT had the advantage of continuity and integrity, which can be macroscopically observed around the bone implant, but due to the presence of metal artifacts, it was not clear enough to detect the osteogenesis between implant-bone interface. Hard tissue section was more complex in the production process. But it was clear to detect the implant bone interface osteogenesis. Conclusion: Micro CT is more suitable for the evaluation of the whole bone around implants. For the implant -bone interface osteogenesis on titanium implant surface, hard tissue section shows more clearer.

Key words: osseointegration, titanium implants, hard tissue section, micro CT

中图分类号:

赖春花，周磊，徐淑兰，郭泽鸿，岳新新. 显微CT与硬组织切片技术在评价牙种植体骨结合中应用的对比研究[J]. 《口腔颌面外科杂志》, 2016, 26(3): 195-201.

LAI Chun-hua, ZHOU Lei, XU Shu-lan, GUO Ze-hong, YUE Xin-xin. A Comparative Study of micro-CT and Hard Tissue Section Technique in the Evaluation of Osseointegration around Dental Implants[J]. 《Journal of Oral and Maxillofacial Surgery》, 2016, 26(3): 195-201.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2016/V26/I3/195

参考文献

[1] Prokharau PA, Vermolen FJ, Garcia-Aznar JM. Model for direct bone apposition on pre-existing surfaces, during peri-implant osseointegration[J]. J Theor Biol, 2012, 304:131-142.

[2] Boyan BD, Schwartz Z. Response of musculoskeletal cells to biomaterials[J]. J Am Acad Orthop Surg, 2006, 14(10 Spec No.):S157-S162.

[3] Davies JE. Bone bonding at natural and biomaterial surfaces[J]. Biomaterials, 2007, 28(34):5058-5067.

[4] 罗亚桐，刘铭，刘勇，等. 戊巴比妥钠与速眠新和戊巴比妥钠对家犬麻醉效果的比较[J]. 湖南师范大学学报（医学版）, 2006, 4(1):78-79.

[5] 赖春花, 周磊, 贾芳, 等. 钛种植体表面早期成骨方式的动物模型研究[J]. 口腔疾病防治, 2016, 24(5):267-272.

[6] Weinlaender M, Kenney EB, Lekovic V, et al. Histomorphometry of bone apposition around three types of endosseous dental implants[J]. Int J Oral Maxillofac Implants, 1992, 7(4):491-496.

[7] Davies JE. Understanding peri-implant endosseous healing[J]. J Dent Educ, 2003,67(8):932-949.

[8] Davies JE. Mechanisms of endosseous integration[J]. Int J Prosthodont, 1998,11(5):391-401.

[9] Aita H, Hori N, Takeuchi M, et al. The effect of ultraviolet functionalization of titanium on integration with bone[J]. Biomaterials, 2009, 30(6):1015-1025.

[10] 吕厚辰, 唐佩福. 骨组织形态及微观结构的影像学评价及进展[J]. 中华骨质疏松和骨矿盐疾病杂志,2016,9(1):68-74.

[11] 郭磊, 赵宏斌. 显微 CT在骨科研究中的应用优势[J]. 昆明医学院学报, 2012, (1B):274-276.

[12] 谢畅, 张宇, 张强. SLA种植体表面喷涂钽形成纳米复合表面预防种植术后早期感染的动物体内实验研究[J]. 中国临床解剖学杂志, 2016, 34(2):208-213.

[13] 钱付民, 宋士霞, 任起辉, 等. 纳米化种植体表面对糖尿病大鼠种植体骨结合影响研究[J]. 中国实用口腔科杂志, 2016, 9(1):31-34.

[14] Ishii K,Matsuo M,Hoshi N, et al. Effect of Ultraviolet Irradiation of the Implant Surface on Progression of Periimplantitis-A Pilot Study in Dogs[J]. Implant Dent, 2016, 25(1):47-53.

[15] Quaranta A, D'Isidoro O,Bambini F, et al. Potential bone to implant contact area of short versus standard implants: an in vitro micro-computed tomography analysis[J]. Implant Dent, 2016, 25(1):97-102.

[16] 吕荣, 王军, 徐新智, 等. 塑料包埋非脱钙骨技术在骨组织学与图像分析中的应用临[J]. 实验病理学杂志, 2006, 22(3):369-370.

[17] Mulder L, Koolstra JH, de Jonge HW, et al. Architecture and mineralization of developing cortical and trabecular bone of the mandible[J]. Anat Embryol(Berl), 2006, 211(1):71-78.

[18] Tamminen IS, Isaksson H, Aula AS, et al. Reproducibility and agreement of micro-CT and histomorphometry in human trabecular bone with different metabolic status [J]. J Bone Miner Metab, 2011, 29(4):442-448.
[19] 王军, 毕龙, 白建萍, 等. 显微CT骨标本扫描的伪影评估与消减控制[J]. 中国体视学与图像分析, 2009, 14(1):85-92.

[20] Schouten C, Meijer GJ, van den Beucken JJ, et al. The quantitative assessment of peri-implant bone responses using histomorphometry and micro-computed tomography[J]. Biomaterials, 2009, 30(27):4539-4549.

[21] Morinaga K, Kido H, Sato A, et al. Chronological Changes in the ultrastructure of titaniumbone interfaces: analysis by light microscopy, transmission electron microscopy, and micro-computed tomography[J]. Clin Implant Dent Relat Res, 2009, 11(1):59-68.

[22] Stoppie N, van der Waerden JP, Jansen JA, et al. Validation of microfocus computed tomography in the evaluation of bone implant specimens[J]. Clin Implant Dent and Relat Res， 2005,7(2):87-94.

[23] Butz F, Ogawa T, Chang TL, et al. Three-dimensional bone-implant integration profiling using micro-computed tomography[J]. Int J Oral Maxillofac Implants, 2006, 21(5):687-695.

[24] Van Oossterwyck H, Duyck J, Vander Sloten J, et al. Use of micro-focus computerized tomography as a new technique for characterizing bone tissue around oral implants[J]. J Oral Implantol, 2000, 26(1):5-12.

选择文件类型/文献管理软件名称

选择包含的内容

显微CT与硬组织切片技术在评价牙种植体骨结合中应用的对比研究

A Comparative Study of micro-CT and Hard Tissue Section Technique in the Evaluation of Osseointegration around Dental Implants

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王静文, 张磊, 苏俭生. 钛表面纳米形貌介导的巨噬细胞极化对骨髓间充质干细胞的影响[J]. 《口腔颌面外科杂志》, 2022, 32(6): 329-335.
[2]	Shahrzad Hosseinijenab, 王静文, 苏俭生, 张磊. 载锶纳米钛表面促进骨质疏松大鼠骨髓间充质干细胞成骨分化的体外研究[J]. 《口腔颌面外科杂志》, 2022, 32(5): 278-283.
[3]	郑凯宁肖月李慕勤张二林姚潍初明慧. 纯镁载聚乳酸-乙醇酸膜层的体内骨结合与降解行为[J]. 《口腔颌面外科杂志》, 2020, 30(5): 279-284.
[4]	冯妍慧芝1（综述）王佐林2（审校）. 牙种植体表面涂层研究进展[J]. 《口腔颌面外科杂志》, 2019, 29(5): 284-289.
[5]	张雨菡，韩雯斐，孟博，苏俭生，肖凡. 调控小鼠腮腺分泌中枢神经系统相关核团的分布[J]. 《口腔颌面外科杂志》, 2019, 29(3): 121-.
[6]	马旭辉，王丽珍，任国欣，吴云腾，顾挺，郭伟. SOX10作为原发性口腔黏膜恶性黑色素瘤分子诊断指标的初步研究[J]. 《口腔颌面外科杂志》, 2019, 29(3): 131-.
[7]	范德生，甄蕾，汪黎明. miRNA-31对糖尿病小鼠来源骨髓间充质干细胞体外成骨分化的影响[J]. 《口腔颌面外科杂志》, 2019, 29(3): 137-.
[8]	郭泽鸿，周磊，徐淑兰，丁祥龙，赵春萍, 卢灿. 改良式骨劈开术水平骨增量对软硬组织的影响[J]. 《口腔颌面外科杂志》, 2019, 29(3): 142-.
[9]	张庆福，牛璐，符国才，聂恒金，吕春堂. 中段桥接式钛合金全下颌骨植入体的设计制作研究[J]. 《口腔颌面外科杂志》, 2019, 29(3): 148-.
[10]	吴东，林兆楠，林彦君，周麟，游冬冬，黄文秀，陈永辉. 自体牙来源骨移植材料在口腔种植中应用的短期临床观察[J]. 《口腔颌面外科杂志》, 2019, 29(3): 152-.
[11]	冯海楠，王淼，常晓峰，杜良智，周慧霞，李晓红，刘少丽，贺龙龙. 上颌窦底提升术后种植修复的十年临床回顾分析[J]. 《口腔颌面外科杂志》, 2019, 29(3): 159-.
[12]	施雄，李生娇，周剑萍，张利，李百龙. 锥形束CT在牙内陷诊断中的应用[J]. 《口腔颌面外科杂志》, 2019, 29(3): 164-.
[13]	岳广娜，王佐林. 石墨烯家族生物相容性的研究进展[J]. 《口腔颌面外科杂志》, 2019, 29(3): 169-.
[14]	孙睿男，王佐林. 单细胞转录组测序技术进展及其在间充质干细胞研究中的应用#br#[J]. 《口腔颌面外科杂志》, 2019, 29(3): 174-.
[15]	周洁, 姜蕾, 赵云富. 一种国产牙种植体5年临床效果评价[J]. 《口腔颌面外科杂志》, 2018, 28(6): 327-331.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

显微CT与硬组织切片技术在评价牙种植体骨结合中应用的对比研究

A Comparative Study of micro-CT and Hard Tissue Section Technique in the Evaluation of Osseointegration around Dental Implants

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价